在重症儿童中快速检测抗生素耐药性基因,使用定制的TaqMan阵列卡
John A Clark1,2, Martin D Curran3, Theodore Gouliouris2,3
1Department of Paediatrics, University of Cambridge, Cambridge CB2 0QQ, UK.
Antibiotics (Basel, Switzerland)
|December 23, 2023
概括
抗微生物耐药性 (AMR) 基因在重症儿童中很常见,即使没有检测到细菌. 一个新的测试,AMR-TAC,可以快速选这些基因,帮助治疗决策.
科学领域:
- 医学微生物学 医学微生物学
- 儿科重症监护 儿科重症监护
- 分子诊断学 分子诊断
背景情况:
- 在重症儿童中,细菌鉴定和抗微生物敏感性测试 (AST) 是缓慢的.
- 抗菌素耐药性 (AMR) 在儿科呼吸道感染中构成了重大挑战.
- 需要快速诊断以指导及时的抗微生物治疗.
研究的目的:
- 为了验证一个定制的抗微生物耐药性基因TaqMan阵列卡 (AMR-TAC) 检测AMR基因.
- 评估AMR-TAC作为重症儿童查工具的可行性.
主要方法:
- 使用已知的耐药分离物开发并验证了一种23个点的AMR-TAC.
- 将AMR-TAC应用于82名机械通风儿童的下呼吸道和便样本.
- 将AMR-TAC结果与表型抗微生物敏感性测试进行比较.
主要成果:
- 在60%的儿童中检测到AMR基因;在17%的儿童中检测到多个基因.
- 在16%的儿童中发现了细菌病原体,其中31%的儿童表现出类型AMR.
- 大多数AMR基因检测与已识别的表型耐药性无关.
结论:
- 在机械通风儿童的样本中经常发现AMR基因,这些儿童怀疑患有呼吸道感染.
- AMR-TAC显示出作为预测抗微生物治疗失败的辅助诊断的潜力.
- 需要进一步的研究来将基因检测与临床结果相关联.
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