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生物工程通过重组氨酸生产酵母的抗菌活性

Sofiya O Pipiya¹, Arsen M Kudzhaev¹, Nisso Z Mirzoeva¹

¹Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.

Antibiotics (Basel, Switzerland)

|December 23, 2023

概括

研究人员对酵母进行了改造,以产生重组氨酸 (rThan),一种强大的抗微生物. 这种生物工程酵母为开发新的生物控制剂提供了一种新的策略,以对抗日益增长的抗生素耐药性.

科学领域:

微生物学微生物学
生物技术是生物技术.
药物发现药物发现药物发现

背景情况:

抗生素耐药性是一个日益严重的全球健康危机,需要新的治疗策略.
抗微生物 (AMP) 由于其独特的机制和低耐药性潜力,显示出作为常规抗生素替代品的希望.
坦纳丁是一种特定的AMP,具有细菌特异性作用和低的真核细胞毒性,使其成为新药开发的强有力的候选者.

研究的目的:

用生物工程来设计一种微生物,用于生产重组氨酸 (rThan).
评估rThan作为对病原细菌的生物控制剂的潜力.
为高水平的 rThan.生产和净化建立最佳条件.

主要方法:

在酵母 *Pichia pastoris* 中,复合氨酸 (rThan) 的异质表达.
为rThan生产优化发酵和净化过程.
对rThan对一系列致病细菌的抗菌活性进行评估.

主要成果:

在 *Pichia pastoris* 中成功地产生了rThan,赋予了抗菌性质.
从培养基中获得高产量,高达20 mg/L的rThan.
证明了rThan对致病细菌的广泛抗菌活性,与合成类似物相比.

结论:

用于rThan生产的生物工程酵母为开发抗菌剂提供了一个新的平台.
开发的方法有助于制造活生物控制剂,以对抗抗生素耐药性.
再组合氨酸对下一代抗生素开发具有重大潜力.

关键词:

抗生素耐药性抗生素耐药性抗微生物类的抗微生物.再组合抗生素是一种复合抗生素.亚酸比酸比酸的方法酵母生物控制剂酵母生物控制剂

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