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评估动物浮游生物的季节和空间动力学通过温带河口的DNA元编码

Jorge Moutinho^1,2, Diego Carreira-Flores^1,2, Pedro T Gomes^1,2

¹Centre of Molecular and Environmental Biology (CBMA) and ARNET-Aquatic Research Network, Department of Biology, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal.

Animals : an open access journal from MDPI

|December 23, 2023

概括

DNA元编码有效地跟踪河口的季节性和空间性动物浮游生物变化. 这种方法通过识别物种,无论其生命阶段如何,来加强监测,揭示社区动态.

科学领域:

海洋生物学海洋生物学
生态生态学生态生态学
遗传学是一个遗传学.

背景情况:

动物浮游生物对河口食物网至关重要,但传统的识别方法具有挑战性.
基于DNA的技术提供了精确的物种识别,克服了形态和发育阶段的局限性.
参考库的完整性对于基于DNA的识别的准确性至关重要.

研究的目的:

用DNA元编码评估利马河口的季节性和空间性动物浮游生物动态.
为了比较两种遗传标记物 (COI和18SrRNA V4) 的有效性,用于动物浮游生物的识别.
评估DNA元编码的潜力,以加强河口监测.

主要方法:

在夏季,秋季和早春期间,DNA元编码应用于来自利马河口的四个地点的动物浮游生物样本.
使用了两个遗传标记物,即细胞染色体c氧化酶子单元I (COI) 和18SrRNA V4区域.
基于测序数据,分析了物种识别和社区组成.

主要成果:

共有327个动物浮游生物物种被确定,两种标记物之间的重叠是最小的 (7%).
季节是物种丰富和组成的主要驱动因素,夏季的多样性最高.
空间变化也影响了社区结构,最下游的地点显示了最多的独家物种.

结论:

季节和空间梯度都对动物浮游生物的丰富性,组成和分类学特征产生重大影响.
DNA元编码非常适合对复杂的动物浮游生物群落进行高密度监测.
这项研究为河口动物浮游生物种群的动态和组成提供了新的见解.

关键词:

DNA元编码的DNA元编码.利马河河口的利马河口.生物监测生物监测沿海生态系统的生态系统细胞染色体c氧化酶子单元I 超变区V4 18S 18S 超变区V4 18S 超变区非土著物种的非土著物种.动物浮游生物 (zooplankton) 是一种动物浮游生物.

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