MiR-217调节SIRT1的表达,促进骨关节炎的炎症和亡反应
Aliki-Alexandra Papageorgiou1, Athanasios Roussos1, Ioanna Papathanasiou1,2
1Laboratory of Cytogenetics and Molecular Genetics, Faculty of Medicine, University of Thessaly, Biopolis, 41500 Larissa, Greece.
Genes
|December 23, 2023
概括
微RNA-217-5p (miR-217-5p) 在骨关节炎 (OA) 冠状细胞中被上调,降调sirtuin 1 (SIRT1) 并导致OA的发病. 抑制miR-217-5p可能为OA提供治疗策略.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 微RNA-217 (miR-217) 的上调与与年龄有关的疾病有关.
- 骨关节炎 (OA) 是一种常见的与年龄相关的退行性关节疾病.
研究的目的:
- 调查miR-217-5p在人体OA冠状细胞中调节sirtin 1 (SIRT1) 的作用.
- 阐明miR-217-5p对OA发病的贡献背后的分子机制.
主要方法:
- 生物信息分析确定SIRT1是miR-217的预测目标.
- miR-217-5p的表达量化在正常和OA胆红细胞中.
- 用miR-217模仿剂/抑制剂和/或针对SIRT1 (siSIRT1) 的siRNA治疗了OA冠状细胞.
- 评估了SIRT1,NF-κBp65和p53乙化,炎症标记物,亡标记物和矩阵调节物的表达水平.
主要成果:
- miR-217-5p在OA胆固醇细胞中显著上调.
- 证实SIRT1是miR-217-5p的直接目标.
- miR-217抑制增加了COL2A1和ACAN的表达,同时降低了促炎和促丧的标志物.
- siSIRT1治疗逆转了miR-217抑制的保护作用.
结论:
- miR-217-5p通过降低SIRT1表达的调节,有助于OA的发病.
- 向miR-217-5p可能代表骨关节炎的潜在治疗方法.
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