相关实验视频

Updated: Jul 7, 2025

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

在转移RNA中使用液体染色学与协同质谱法 (LC-MS/MS) 结合的子[a]烯附加物进行表征

Cassandra Herbert¹, Corinna L Ohrnberger¹, Ella Quinlisk¹

¹Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, 301 Clifton Court, Cincinnati, OH 45221-0172, USA.

|December 23, 2023

概括

二醇环氧化物 (BPDE) 是一种污染物,可以破坏DNA和蛋白质. 这项研究确定了BPDE附加的核糖体和转移RNA (tRNA),揭示了BPDE-RNA相互作用的新见解.

科学领域:

环境科学环境科学
分子生物学分子生物学
生物化学生物化学

背景情况:

环境污染物[a]烯 (B[a]P) 和其活性形式[a]二醇环氧化物 (BPDE) 是已知的突变原体,它们会损害DNA和蛋白质.
对BPDE对核糖核酸 (RNA) 的影响尚不清楚,这代表了毒理学和分子生物学方面的重大知识差距.

研究的目的:

为了研究[a]二醇环氧化物 (BPDE) 在核糖核酸 (RNA) 上的潜在形成和特征.
识别新型BPDE-RNA adducts并了解它们对RNA结构和功能的影响.

主要方法:

核糖核酸标准和真核转移RNA (tRNA) 与[a]二醇环氧化物 (BPDE) 的反应.
反应产品的表征使用液态染色学与双重质谱学 (LC-MS/MS) 相结合.
分离和分析修饰和添加的核糖核酸.

主要成果:

几种[a]二醇环氧化物 (BPDE) 衍生物的转录后修饰被确定在核糖核酸上.
这项研究成功地检测并描述了转移RNA (tRNA) 上形成的BPDE附加物.
开发的分析方法可以识别多种BPDE-RNA adducts.

结论:

这项研究提供了首次全面识别BPDE-RNA添加物,包括对核糖核酸和tRNA的修改.
这些发现突出了BPDE修改RNA的潜力,影响其结构和功能.
建立的方法可用于研究环境污染物对RNA完整性和细胞过程的更广泛影响.

关键词:

在 BPDEDE 中,B[a]PP 的意思是引进的RNA是RNA的添加物.添加组的添加组是什么?修改后的核化物转录后的修改转录后的修改

更多相关视频

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

相关概念视频

Mass Spectrometry: Aromatic Compound Fragmentation

Mass Spectrometry: Aromatic Compound Fragmentation

Upon ionization, aromatic compounds generate a molecular ion that is observed as a prominent peak in their mass spectra. For example, the molecular ion peak for benzene appears at a mass-to-charge ratio of 78, while toluene is observed at a mass-to-charge ratio of 92. The molecular ion benzene is highly stable and does not readily undergo further fragmentation due to the significant amount of energy required to disrupt the aromatic stability of the benzene ring. In contrast, the molecular ion...