在adipokinetic荷尔蒙中托芬和proline的氧化产物-人工制品或翻译后的修改?
Simone König1, Heather G Marco2, Gerd Gäde2
1IZKF Core Unit Proteomics, Interdisciplinary Center for Clinical Research, University of Münster, Röntgenstr. 21, 48149 Münster, Germany.
脂肪动力荷尔蒙 (AKHs) 中的基似乎是昆虫中的自然修饰,而不是人工制造. 这一发现得到了ex vivo释放实验和生物测试的支持,这表明了内源性过程.
科学领域:
- 昆虫生理学 昆虫生理学
- 内分泌学 在内分泌学.
- 生物化学 生物化学
背景情况:
- 脂肪动力激素 (AKHs) 是关键的昆虫调节生理过程.
- 已知AKHs的特征是阻塞的末端,trp在8位,通常是proline在6位.
- 很少报告过像氧 ([Hyp6) 和kynurenine这样的翻译后修改.
研究的目的:
- 调查AKH中氧和金氨酸的修饰是自然的还是人造的.
- 确定昆虫心脏体 (CC) 中含有氧氨酸的AKHs的患病率.
主要方法:
- 使用液体染色学高分辨率质谱法 (LC-MS) 来分析昆虫CC提取物中的AKHs.
- 在体内代谢性高三血性试验中测试合成[Hyp6]-AKH.
- 从 *Periplaneta americana* 和 *Blaberus atropos* 的新切割的 CC 中释放 AKH 的活体分析.
主要成果:
- 在的十酸中检测到酸 ([Hyp6) 存在于低度 (<7%).
- 在新鲜的CC中发现了来自*B. atropos*的[Hyp6]-AKHs,并在体外释放出来,证实了它们的内源性质.
- 在白 (*Kalotermes flavicollis*) 和子 (*Tetrix subulata*) 的 AKH 中也发现了素.
结论:
- 虽然Trp氧化很可能是样品处理的工件,但基化表明昆虫的自然,内源性过程.
- 实验证据支持氧作为昆虫AKHs.的真正的翻译后修饰.
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