生物特征和全基因组分析Bacillus subtilis MG-1从明克便样本中分离出来的
Jianwei Ren1, Detao Yu1, Nianfeng Li1
1College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Microorganisms
|December 23, 2023
概括
从子中分离出来的 Bacillus subtilis MG-1 在恶劣条件下表现出强大的生存能力和素水解活性. 全基因组测序揭示了它在动物健康应用中的益生菌潜力.
科学领域:
- 微生物学 微生物学
- 益生菌 益生菌 益生菌
- 动物科学动物科学
背景情况:
- 细菌细菌是一种关键的肠道微生物和常见的益生菌.
- 的肠道微生物群可以容纳有益的细菌菌株.
- 评估新型益生菌候选者对于动物健康至关重要.
研究的目的:
- 评估来自子的Bacillus subtilis MG-1的生物特征和益生菌潜力.
- 为了优化B. subtilisMG-1原油提取物的素水解.
- 进行全基因组测序以进行全面分析.
主要方法:
- 从子便样本中分离和描述B. subtilis MG-1.
- 在低pH值,肠液和胃酸条件下评估生存率.
- 通过原始细菌提取物优化素水解.
- 使用PacBio平台进行全基因组测序和随后的基因注释 (KEGG,蛋-NOG,GO).
主要成果:
- 在pH 2下,B. subtilis MG-1的生存率很高 (64.75%),肠液和胃酸的生存率提高.
- 原始提取物显示出显著的素化活性,优化至32.04U/mL.
- 基因组分析揭示了代谢途径的丰富性,并确定了77个与传送器相关的基因.
- 与能量,新陈代谢,转录和细胞生物发生相关的基因丰富.
结论:
- B. subtilis MG-1 具有显著的益生菌特性,包括环境耐受性和酶活性.
- 基因组测序提供了对其代谢能力和潜在功能的洞察.
- B. subtilis MG-1 显示出作为促进动物健康的益生菌添加剂的前景.
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