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对抗Vibrio alginolyticus的菌体VA5的分离和表征

Qingfang Hao^1,2, Yue Bai^1,2, Haolong Zhou³

¹Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China.

|December 23, 2023

概括

体VA5有效控制水产养殖中的致病细菌. 这种菌体表现出广泛的宿主范围和稳定性,使其成为水产品种植的有希望的生物控制剂.

科学领域:

微生物学微生物学
水产养殖是水产养殖的一种方式.
生物技术是生物技术.

背景情况:

菌体 (菌体) 是感染细菌的病毒.
菌体为控制水产养殖中的致病细菌提供了一个自然的替代方案.
由Vibrio物种引起的肌病对水生生物构成重大威胁.

研究的目的:

孤立和描述一种用于控制Vibrio alginolyticus的新型细菌菌体.
评估隔离的菌体作为水产养殖中的生物控制剂的有效性.

主要方法:

从水产养殖环境中分离菌体VA5,使用双层亚格板方法与Vibrio alginolyticus.
基因组测序,形态观测和分析菌体VA5的生物特性.
对培养中的Vibrio alginolyticus的菌体VA5的疗效的评估.

主要成果:

菌体VA5被分离出来,被描述为一种长尾菌体,具有圆形的dsDNA基因组 (35,866bp,46%G+C含量).
菌体VA5的最佳条件包括MOI的1,20分钟化,30分钟爆发和92.26 PFU/细胞分裂.
体VA5在-20°C,70°C和pH值2-10时表现出稳定性,并显著抑制了中的V. alginolyticus.

结论:

体VA5是一种强大的菌体,具有广泛的宿主范围.
菌体VA5显示出作为水产养殖中的Vibrio alginolyticus生物控制剂的巨大潜力.

关键词:

生物特征生物特征生物控制生物控制双石板方法的双石板方法.进行全基因组测序.

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