斑马鱼的调节 (Danio rerio) 肠粘膜屏障功能 养不同的后生物和来自乳杆菌的益生菌
Mark Rawling1, Marion Schiavone2, Amélie Mugnier2
1Aquatic Animal Nutrition and Health Research Group, School of Marine and Biological Sciences, Plymouth University, Plymouth, Devon PL4 8AA, UK.
Microorganisms
|December 23, 2023
概括
饮食中的益生菌和后生菌,包括非活化的乳酸菌种,增强斑马鱼的肠道屏障功能和免疫力. 这些干预措施加强了肠道内膜,并以特定菌株的方式调节免疫反应.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 斑马鱼模型的模型
背景情况:
- 肠道微生物对于肠道屏障功能至关重要.
- 益生菌和后生菌是肠道疾病的潜在治疗剂.
- 了解菌株特异性影响是针对性干预的关键.
研究的目的:
- 评估特定的乳杆菌菌株 (活体和无活体) 对斑马鱼肠道屏障功能和免疫力的影响.
- 为了确定益生菌或后生菌制剂是否提供对肠道功能障碍的保护作用.
- 调查乳酸菌干预的菌株特异性和过程依赖性影响.
主要方法:
- 斑马鱼被养的饮食补充了活的*Lactobacillus helveticus*,非活化的*Lactobacillus helveticus*和非活化的*Lactobacillus plantarum*在6 × 10^6细胞/g.
- 对肠长度,内皮淋巴细胞 (IELs),杯状细胞 (GCs),抗菌 (AMP) 生产和紧结蛋白表达 (zona-occludin 2a) 的评估影响.
- 分析了自适应性免疫反应,包括CD8+细胞计数和关键细胞因子的基因表达 (例如*il22*, *ifnγ*).
主要成果:
- 这三种治疗方法 (益生菌和后生菌) 都以一种取决于菌株和过程的方式加强了肠道屏障功能和先天免疫力.
- 观察到肠道小的长度增加,IEL比例,GC群体,AMP产生和zo-2a表达.
- LPPost (非激活的*Lactobacillus plantarum*) 调节了适应性免疫力,可能驱动Th17/ILC3和Th1/ILC1反应,由改变的基因表达和免疫细胞群体表明.
结论:
- 通过饮食中服用非活化的*乳酸菌种*是一种增强肠道屏障功能的有希望的策略.
- 这些干预措施可以以特定菌株的方式调节肠道免疫反应.
- 这些发现支持了特定益生菌和后生菌制剂改善肠道健康和免疫调节的潜力.
相关概念视频
01:22Probiotics
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
01:18Functions of the Gut Microbiota
The gut microbiota includes trillions of microorganisms that colonize the human gastrointestinal tract, including bacteria, archaea, viruses, and fungi. This complex ecosystem plays a critical role in maintaining intestinal and systemic health. Most of these microbes inhabit the large intestine, establishing a relatively stable and diverse community that contributes to gut homeostasis through various metabolic, immunological, and protective mechanisms.Dominant bacterial phyla, such as...
01:18Dysbiosis of the Gut Microbiota
The human gut microbiome includes a diverse array of microbial species, including beneficial commensals and opportunistic pathogens, which interact to support host health. These microbes contribute to essential functions such as nutrient metabolism, immune system modulation, and maintenance of intestinal barrier integrity. However, disruptions to this equilibrium—referred to as dysbiosis—can have widespread physiological consequences.Dysbiosis is often characterized by reduced microbial...
01:21Microbiota Modulation by Antibiotics
Antibiotics have revolutionized modern medicine by saving countless lives from bacterial infections. However, their widespread use has inadvertently harmed the delicate balance of the human gut microbiota. The gut microbiota, a complex community of bacteria, archaea, viruses, and fungi, plays a vital role in regulating metabolism, immune responses, and maintaining intestinal health. Antibiotics, especially broad-spectrum types, disrupt this ecosystem by eradicating both harmful and beneficial...