由Leishmania (Leishmania) amazonensis释放的细胞外囊,具有明显毒性特征的前列腺细胞,以不同的方式调节巨细胞功能
Rogéria Cristina Zauli1, Isabelle Carlos de Souza Perez2, Aline Correia Costa de Morais1
1Programa de Pós-Graduação Biologia-Química, Instituto de Ciências Ambientais Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema 04021-001, SP, Brazil.
Microorganisms
|December 23, 2023
概括
来自实验性感染的Leishmania amazonensis寄生虫,与长期培养的不同,增加了巨细胞感染和炎症反应. 长期培养的寄生虫的细胞外囊泡也增加了巨细胞对Leishmania感染的易感性.
科学领域:
- 寄生虫学的寄生虫学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 莱什曼尼亚种类 (Leishmania spp.) 是一种 导致利什曼病,这是一种在发展中国家普遍存在的疾病.
- 寄生虫的毒性因素会破坏巨细胞的功能和免疫反应.
- 来自Leishmania的细胞外囊泡 (EVs) 可以调节巨细胞激活和疾病进展.
研究的目的:
- 研究Leishmania amazonensis promastigotes从实验性感染与长期培养对巨细胞行为的差异性影响.
- 确定从这些独特的寄生虫培养中获得的EVs对巨细胞反应的影响.
主要方法:
- 在Leishmania amazonensis promastigotes中对lpg3和gp63的基因表达分析.
- 使用来自实验性感染 (IVD-P) 和长期培养 (LT-P) 的寄生虫进行巨感染测定.
- 用来自LT-P的EV治疗巨细胞,然后进行寄生虫挑战和细胞因子分析.
主要成果:
- 与LT-P相比,IVD-P显示lpg3和gp63基因的表达显著更高.
- 感染IVD-P的巨细胞表现出更高的感染率和寄生虫内部化.
- 来自LT-P治疗的巨细胞的EV显示,在寄生虫挑战时,感染百分比和炎症性细胞因子产生增加.
- 电子药物没有减少感染的巨细胞中的寄生虫负载,也没有与参考药物表现出协同作用.
结论:
- 来自实验性感染和长期培养的Leishmania amazonensis寄生虫对巨细胞的反应产生不同的影响.
- 长期培养寄生虫产生的EVs在早期感染阶段会影响巨细胞的反应.
- 需要进一步的研究,以了解寄生虫衍生的EVs在莱什曼病原体的特定作用.
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