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对不同M的宿主蛋白质组的时间分析. 肺结核菌株揭示延迟表观遗传编排

Prabhakar Babele¹, Mukul K Midha², Kanury V S Rao¹

¹Translational Health Science and Technology Institute, Faridabad 121001, India.

|December 23, 2023

概括

结核病细菌通过巨细胞中复杂的蛋白质变化来逃避宿主防御. 这项研究揭示了菌株特异性和时间依赖性宿主反应,有助于开发新的结核病治疗方法.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
蛋白质组学是指蛋白质组学.

背景情况:

结核病 (TB) 仍然是全球主要的细菌死亡原因.
由Mycobacterium tuberculosis (MTB) 引起的宿主免疫逃避涉及表观遗传机制.
了解宿主-病原体相互作用对于开发有效的结核病疗法至关重要.

研究的目的:

在感染不同Mycobacterium结核菌菌株期间研究巨类宿主细胞的时间蛋白质动力学.
为了识别由MTB感染重新校准的宿主防御机制.
提供对宿主对毒性和毒性MTB菌株的反应的全面蛋白质学概况.

主要方法:

巨类细胞被实验室 (H37Ra,H37Rv) 和临床 (BND433,JAL2287) MTB菌株感染了6,18,30和42小时.
使用SWATH-MS.进行了定量蛋白质组分析.
质谱数据被存储在ProteomeXchange联盟 (PRIDE,PXD022352) 中.

主要成果:

早期感染 (6h) 显示了与氧化应激和细胞骨相关的蛋白质.
中期和晚期阶段 (18-42小时) 显示了蛋白质的变化,这些变化涉及到基因稳定性,基因表达和蛋白质贩运.
在宿主蛋白质组中观察到显著的菌株特异性和时间依赖的变异.

结论:

宿主对MTB感染的蛋白质反应是动态的,并取决于菌株.
蛋白质组的洞察力可以为结核病的宿主导治疗和诊断的发展提供信息.
这项研究提供了在MTB感染期间对宿主防御再校准的全面蛋白质学景观.

关键词:

在 LFQ 蛋白质组学.结核病菌菌菌的结核病菌的结核病菌.这就是SWATH-MS.类似巨细胞的THP1细胞时间性蛋白质组学

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