在城市医院重症监护室爆发了抗万科米辛抗性肠球菌:抗性的分子表征
Feray Ferda Şenol1, Elif Seren Tanrıverdi2, Özlem Aytaç1
1Microbiology Laboratory Unit, Elazığ Fethi Sekin City Hospital, 23280 Elazığ, Turkey.
Medicina (Kaunas, Lithuania)
|December 23, 2023
概括
抗万科胺素抗性肠球菌 (VRE) 在重症监护室迅速传播,有88.8%的隔离物表现出克隆相似性. 这凸显了联合流行病学和分子分析的必要性,以控制VREfm疫情.
科学领域:
- 传染性疾病 传染性疾病
- 微生物学 微生物学
- 流行病学 流行病学
背景情况:
- 抗胺素抗性肠球菌 (VRE) 是重要的医院病原体.
- VRE殖民和感染对住院患者构成威胁,特别是在重症监护机构.
- 抗万科胺素耐药的Enterococcus faecium (VREfm) 的出现需要详细追踪其传播.
研究的目的:
- 阐明VREfm在重症监护病房中的逐步传播.
- 在明显的流行病期间调查VREfm分离物的克隆相关性.
- 为了确定VRE殖民和特定耐药基因的流行率.
主要方法:
- 从重症监护病房患者收集的直肠拭子样本来确定VRE殖民.
- 聚合酶链反应 (PCR) 用于在VREfm分离物中检测VanA和VanB耐药基因.
- 任意启动PCR (AP-PCR) 和脉冲场凝电泳 (PFGE) 用于克隆关联分析.
主要成果:
- 在2601个直肠擦拭样本中,在61个样本中检测到VRE;VREfm是占主导地位的物种 (85.52%).
- 所有54个VREfm分离物都携带了vanA基因;克隆分析显示了12种基因型,聚类率为88.8%.
- VREfm分离体对多种抗生素具有很高的耐药性,包括安皮西林,勒沃富洛克萨和泰克奥普拉宁.
结论:
- 该研究表明,VREfm分离物之间存在高度的克隆相似性,这表明流行病正在蔓延.
- 结合流行病学和分子分析对于有效了解和管理VREfm疫情至关重要.
- VREfm感染导致长时间住院,增加成本和死亡率,这凸显了问题的严重性.
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