核桃绿皮提取物的抗菌活性和机制
Lei Wang1, Wenjing Li1, Xuanyue Li1
1Xinjiang Key Laboratory of Herbivore Nutrition for Meat & Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China.
Molecules (Basel, Switzerland)
|December 23, 2023
概括
核桃绿皮 (WGHs) 提取物对常见病原体具有强大的抗菌活性. 这项研究揭示了WGHs损害细菌细胞膜并干扰能量代谢,突出了它们作为天然抗生素替代品的潜力.
科学领域:
- 自然产品 化学 化学
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 核桃绿皮 (WGHs) 是农业副产品,具有潜在的抗菌特性.
- 由于抗菌素耐药性日益增加,人们越来越需要替代传统抗生素.
研究的目的:
- 调查WGH提取物对关键细菌病原体的抗菌活性.
- 阐明WGH (WGHa) 无水乙醇提取物的抗菌机制.
主要方法:
- 抗菌活性测定对*黄金葡萄球菌*,*细菌*和*大肠杆菌*.
- 超高性能液态染色学-并联质谱学 (UHPLC-MS/MS) 用于化合物识别.
- 生物化学分析和传输电子显微镜 (TEM) 用于机制分析.
- 转录组测序用于基因表达造型.
主要成果:
- WGHa对所有测试的细菌都表现出显著的抑制作用.
- 在WGHa中发现的主要活性化合物包括类,类和黄类.
- 治疗WGHa导致细胞膜透性增加,细胞内成分泄漏,ATP水平降低,细胞变形.
- 转录组分析揭示了RNA降解,翻译,能量代谢和细胞壁相关途径的变化.
结论:
- 通过破坏细菌细胞膜和细胞壁,WGHa表现出广泛的抗菌活性.
- WGHa干扰细菌的能量代谢,RNA完整性和DNA复制.
- 这些发现支持WGHs作为新型抗菌剂和抗生素替代品的来源的潜力.
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