通过Bacillus subtilis的固态发酵修改的大豆粉蛋白的结构特征和特性
Xinyu Miao1, Honghong Niu1, Mubai Sun1
1Institute of Agro-Food Technology, Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 133000, China.
Molecules (Basel, Switzerland)
|December 23, 2023
概括
使用Bacillus subtilis对大豆粉 (SBM) 的固态发酵改善了其蛋白质特性. 这种修改减少了反营养因素,并增强了SBM.
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 蛋白质化学 蛋白质化学
背景情况:
- 由于高分子量和反营养因素,大豆粉 (SBM) 蛋白具有有限的应用.
- 修改SBM蛋白质对于其作为食品成分的更广泛使用至关重要.
研究的目的:
- 为了优化SBM的固态发酵,使用Bacillus subtilis.
- 为了研究SBM蛋白在发酵后的结构和性质变化,用于食品应用.
主要方法:
- 用优化的参数进行SBM固态发酵 (37°C,12%的注射剂,47小时,1:0.58的物质-液体比).
- 分析蛋白质结构 (二级结构,SDS-PAGE),粒子大小,泽塔潜力和表面形态 (SEM,CLSM).
- 评估蛋白质溶解性,疏水性,自由硫含量和抗原性.
主要成果:
- 优化发酵显著改善了酸溶性蛋白质含量.
- 发酵改变了蛋白质的二次结构,减少了大分子量带,并产生了较小的.
- 修改后的SBM (FSBM) 呈现出可溶性增加,疏水性降低,抗原性降低,以及多孔的表面结构.
结论:
- 固态发酵有效地修改SBM蛋白质,增强其功能性质.
- 改善的FSBM蛋白质特性促进其作为一种有价值的食品成分的使用.
- 这一过程为食品工业应用中升级SBM提供了一种可行的方法.
相关概念视频
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
Production of Antibiotics
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
Production of Biopesticides
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...


