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碳纳米结构对抗SARS-CoV-2 S-蛋白抗体的免疫感应

Jarid du Plooy¹, Branham Kock¹, Nazeem Jahed¹

¹SensorLab, Department of Chemistry, University of the Western Cape, Robert Sobukwe Road, Bellville, Cape Town 7535, South Africa.

Molecules (Basel, Switzerland)

|December 23, 2023

概括

这项研究开发了一种石墨烯量子点 (GQD) 免疫传感器,用于检测SARS-CoV-2 IgG抗体. 便携式生物传感器提供灵敏和选择性检测,对于在资源有限的环境中监测疫苗疗效至关重要.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
免疫感知是一种免疫感知.

背景情况:

随着COVID-19的流行,人们需要快速,负担得起的诊断和疫苗有效性监测.
电化学免疫传感为传统方法提供了一种灵敏,便携的替代方案.
石墨烯量子点 (GQDs) 显示出提高生物传感器灵敏度的前景.

研究的目的:

开发一个GQD功能化的电化学免疫传感器,用于检测SARS-CoV-2 IgG抗体.
为资源有限的设置创建一个便携式,具有成本效益的诊断工具.
为了能够监测疫苗诱导的免疫反应和抗体寿命.

主要方法:

使用GQDs功能化的印碳电极 (SPCE) 的制造.
针对抗体捕获的SARS-CoV-2尖端蛋白RBD抗原的固定.
使用氧化还原探针对免疫复杂形成的电化学检测.
优化GQD度,EDC/NHS化学和化参数.

主要成果:

GQD功能化的免疫传感器选择性地检测到从0.5到100ng/mL的抗SARS-CoV-2IgG抗体.
达到2.028 ng/mL的检测极限 (LOD),回归率高 (0.9599).
与其他碳纳米材料相比,证明了卓越的性能.

结论:

开发的GQD免疫传感器是针对抗SARS-CoV-2IgG检测的敏感和选择性工具.
这种便携式生物传感器可以帮助监测疫苗的疗效和免疫反应.
这项技术有可能在资源有限的环境中用于护理点诊断.

关键词:

这就是SARS-CoV-2病毒.碳纳米材料的碳纳米材料电化学免疫传感器电化学免疫传感器没有标签的检测检测.医疗服务中心的设备.

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Conjugated Proteins

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