相关实验视频

Updated: Jul 7, 2025

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

Published on: July 28, 2015

酸盐 (Pi) 输送器 PIT1 诱导Salmonella中的Pi饥饿 - - 在HeLa细胞中含有真空细胞

Wen Yang^1,2, Yingxing Feng^1,3, Jun Yan^1,2

¹The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071, China.

International journal of molecular sciences

|December 23, 2023

概括

沙门氏菌Typhimurium操纵宿主免疫力,降低真空体内的酸盐水平,促进其复制. 这涉及通过TLR4-MyD88-NF-κB通路对PIT1进行上调,影响毒性和细菌存活.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

沙门氏菌 (Salmonella enterica serovar Typhimurium,S. Typhimurium) 是一种引起胃肠道疾病的食源性病原体.
S. Typhimurium居住在宿主细胞内的含沙门氏菌真空体 (SCV),体验低酸盐 (Pi) 度.
宿主细胞降低SCV中的Pi的机制以及S. Typhimurium如何感知并对此做出反应仍然不清楚.

研究的目的:

阐明宿主细胞在S. Typhimurium感染期间降低SCV中的酸盐度的机制.
调查TLR4-MyD88-NF-κB信号通路和PIT1在SCVs内的Pi恒温中的作用.
了解S. Typhimurium如何利用宿主免疫反应进行细胞内复制.

主要方法:

免疫光学和西部涂抹被用于分析SCV膜上的PIT1定位.
用基因沉默和过度表达技术来研究PIT1.1的功能.
进行了沙门氏菌病原性岛-2 (SPI-2) 基因表达和SCV-溶解体融合的分析.

主要成果:

S. Typhimurium通过TLR4-MyD88-NF-κB通路对宿主细胞PIT1进行上调,从而导致Pi从SCV中运输出来,并导致Pi饥饿.
PIT1定位在SCV膜上,其调制影响Pi饥饿,SPI-2基因表达和SCV内的细菌复制.
破坏TLR4-MyD88-NF-κB通路或S. Typhimurium ΔmsbB突变抑制了SPI-2表达并增强了SCV-lysosome融合.

结论:

S. Typhimurium利用宿主天生的免疫信号 (TLR4-MyD88-NF-κB) 通过PIT1调节细胞内Pi水平,促进其生存和复制.
这种机制突显出一种新的宿主-病原体相互作用,其中宿主免疫反应无意中促进了细菌细胞内增殖.
这些发现为开发针对细菌感染的广泛疗法提供了潜在的目标,通过干扰这种Pi介导的复制策略来对抗细菌感染.

关键词:

在 PIT1 中,PIT1 是 PIT1.的饥饿是因为Pi的饥饿.沙门氏菌进入的血清 Typhimurium 类型.沙门氏菌病原性岛-2 含有沙门氏菌的真空孔中含有沙门氏菌.

更多相关视频

Application of Fluorescent Nanoparticles to Study Remodeling of the Endo-lysosomal System by Intracellular Bacteria

Application of Fluorescent Nanoparticles to Study Remodeling of the Endo-lysosomal System by Intracellular Bacteria

Published on: January 2, 2015

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Published on: August 11, 2014

相关概念视频

ATP Driven Pumps III: V-type Pumps

ATP Driven Pumps III: V-type Pumps

V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...