维塞宁-2对热和氧化应激引起的蛋白质结构损伤的抗聚合和保护作用
Giuseppe Tancredi Patanè1, Lisa Lombardo1, Stefano Putaggio1
1Department of Chemical, Biological, Pharmaceutical and Environmental Science, University of Messina, 98166 Messina, Italy.
International journal of molecular sciences
|December 23, 2023
概括
维塞宁-2是一种C-glycosyl flavonoid,可以防止热引起的人体血清白蛋白 (HSA) 聚合,并防止某些自由基. 它与HSA结合在一起.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 黄类研究 黄类研究
背景情况:
- 维塞宁-2是一种具有罕见的C-糖链接的黄类化合物.
- 有证据表明,C-glycosyl黄类药物可能在没有脱糖化的情况下被吸收.
- 人体血清白蛋白 (HSA) 是一种关键蛋白质,参与运输,易受聚合和氧化损伤.
研究的目的:
- 研究vicenin-2对人类血清白蛋白 (HSA) 的抗聚合和抗氧化特性.
- 阐明vicenin-2和HSA之间的结合机制和分子相互作用.
主要方法:
- 在体外测试以评估抗聚合和抗氧化作用.
- 光谱技术包括紫外线可见,圆形二极化和光光谱.
- 用于相互作用分析的分子建模.
主要成果:
- 维西宁-2抑制了热引起的HSA纤维化和聚合 (0.1-25.0μM).
- 维西宁-2提供了对超氧化离子和基激素的保护,而不是活性.
- 谱学和建模数据证实vicenin-2与HSA在Sudlow的部位I的相互作用,涉及疏水性,Pi-pi堆叠和结合相互作用,导致α-螺旋结构减少.
结论:
- 维西宁-2具有与HSA相关的显著抗聚合和抗氧化活性.
- 维西宁-2与HSA的结合涉及Sudlow的部位I的特定分子相互作用.
- 这些发现强调了vicenin-2在抵消蛋白质聚合和氧化应激方面的潜在治疗应用.
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