希斯提丁结合的丁铁复合物:抗氧化和抗激素特性
Konstantin B Shumaev1, Olga V Kosmachevskaya1, Elvira I Nasybullina1
1Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.
International journal of molecular sciences
|December 23, 2023
概括
用L-卡诺辛清除活性氧和物种的丁铁复合物 (DNIC),作为抗氧化剂. 血清白蛋白结合的DNICs还可以保护脂质免受氧化损伤,这表明它们在细胞保护中扮演着不同的角色.
科学领域:
- 生物化学 生物化学
- 自由基化学 自由基化学
- 氧化压力研究研究 氧化压力研究
背景情况:
- 丁铁复合物 (DNIC) 是具有显著生物活性的关键氧化衍生物.
- DNICs的抗氧化和抗根性质具有重要的生理重要性.
- 了解DNIC在氧化应激下与益氧化剂的相互作用至关重要.
研究的目的:
- 研究与L-卡诺辛和血清白蛋白结合的DNICs的抗氧化和抗激素活性.
- 阐明这些DNIC与各种活性氧和物种相互作用的机制.
- 评估DNICs对生物系统中氧化损伤的保护作用.
主要方法:
- 研究了DNIC与益氧化剂 (三丁氧化水,铁离子,安吉利盐) 的相互作用,使用L-carnosine和牛血清白蛋白作为配体.
- 评估了对皮佩拉津基和超氧化离子基 (山丁/山丁氧化酶系统) 的激素清除活性.
- 评估了对oxoferryl髓质球蛋白和辅酶Q9/Q10的氧化降解在老鼠心肌同质化的影响.
主要成果:
- 卡诺辛结合的DNIC有效地减少了皮佩拉的自由基,并与超氧化基发生反应.
- 卡诺辛DNICs降低了肌球蛋白的oxoferryl形式.
- 血清白蛋白结合的DNICs虽然不太稳定,但抑制了辅酶Q9和Q10的氧化降解.
结论:
- DNICs,特别是那些含有L-carnosine的DNICs,表现出强大的抗氧化和抗根性质.
- 肉和白蛋白DNIC都显示出对氧化损伤的保护作用,尽管通过不同的机制和稳定性.
- 这些发现凸显了DNIC在管理反应性物种和减轻氧化应激方面的多方面的作用.
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