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人类炎症性皮肤疾病的ex vivo模型的开发

Eddy Hsi Chun Wang¹, Rebecca Barresi-Thornton¹, Li-Chi Chen²

¹L'Oreal Research and Innovation, Clark, NJ 07066, USA.

International journal of molecular sciences

|December 23, 2023

概括

新的ex vivo人体皮肤模型显示,炎症性皮肤病会破坏皮肤屏障脂质. 这些模型模仿人类皮肤炎症,有助于开发针对皮肤损伤的新疗法.

科学领域:

皮肤病学皮肤病学
皮肤生物学皮肤生物学
炎症研究炎症研究

背景情况:

传统的炎症性皮肤病研究使用有限的动物和重建的人类表皮模型.
现有的模型无法完全复制人类皮肤的复杂性和病理性脂质特征变化.

研究的目的:

开发模拟人类炎症性皮肤状况的先进实验模型.
为了研究皮肤形态,细胞变化和脂质组概况,以应对炎症触发因素.

主要方法:

使用人体活体皮肤扩展剂,用触发剂刺激,以模拟血管生成,刺激和慢性T细胞激活.
评估了形态,细胞 (巨细胞,巨细胞,白细胞) 和细胞因子的改变.
进行了皮肤上脂质学分析,以分析胺子类和乙烯酸链长度.

主要成果:

亲血管新生模型显示杆细胞脱粒和血管新生因素增加.
刺激性和慢性炎症模型显示表皮干扰,免疫细胞透和细胞因子升高.
在刺激性/慢性模型中观察到基本胺和较短的乙烯链 (

结论:

开发了针对炎症性皮肤疾病的新型人体体外活体模型.
在对刺激和慢性炎症的反应中显示出显著的皮肤屏障脂质变化.
模型对免疫抑制剂有反应,显示出治疗开发的潜力.

关键词:

在ex vivo模型中使用.皮肤障碍物皮肤障碍物皮肤炎症皮肤炎症

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