藻类Uronema belkae具有两种结构类型的[FeFe]-化酶,具有不同的生化特性
Ghazal Alavi1, Vera Engelbrecht1, Anja Hemschemeier1
1Faculty of Biology and Biotechnology, Photobiotechnology, Ruhr University Bochum, 44801 Bochum, Germany.
International journal of molecular sciences
|December 23, 2023
概括
藻类Uronema belkae*使用细菌和藻类[FeFe]-酶酶产生 (H2). 这些独特的酶在藻类的代谢途径中被不同的方式集成.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 光合作用 光合作用
背景情况:
- 微藻利用与光合作用相关的[FeFe]-基酶将光能转化为分子 (H2).
- 细菌[FeFe]-基酶具有H和F域,而大多数藻类类型是M1型 (仅H域),与ferredoxin PetF.相互作用.
研究的目的:
- 为了研究[FeFe]-基酶在化藻*Uronema belkae*中的存在和功能.
- 描述Uronema belkae*中发现的不同类型的[FeFe]-基酶及其代谢整合.
主要方法:
- 基因转录分析.
- 在低氧条件下测试气生产量.
- [FeFe]-酶酶的生物化学表征.
主要成果:
- *Uronema belkae* 拥有细菌类和藻类类[FeFe]-酶基因,这些基因被转录.
- 在低氧条件下,藻类产生H2.
- 生物化学分析证实了两种酶类型的不同特征,其中细菌类型的酶与PetF.不相互作用.
结论:
- *Uronema belkae*使用两个不同的[FeFe]-酶系统来生产H2.
- *U. belkae*中的细菌型酶与典型的藻类M1型酶相比,具有不同的相互作用方式.
- 这表明,酶酶在藻类中的代谢整合差异.
相关概念视频
Allosteric Proteins-ATCase
5.7K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.7K
Protein Complexes with Interchangeable Parts
2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K
Reduction of Alkenes: Asymmetric Catalytic Hydrogenation
3.3K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.3K
Ligand Binding and Linkage
4.8K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.8K
Isomerism in Alkenes
12.0K
Alkenes like 1-butene and 2-butene exhibit constitutional isomerism, as they differ in the position of the double bond. Further, 2-butene exhibits stereoisomerism and exists as two distinct compounds differing in spatial arrangement.
An isomer is called cis-2-butene when the methyl groups are on the same side of the double bond, and the other stereoisomer, in which methyl groups are on the opposite side of the double bond, is called trans-2-butene. The cis and trans stereoisomers are not...
An isomer is called cis-2-butene when the methyl groups are on the same side of the double bond, and the other stereoisomer, in which methyl groups are on the opposite side of the double bond, is called trans-2-butene. The cis and trans stereoisomers are not...
12.0K
Constitutional Isomers of Alkanes
18.2K
Organic compounds of the same molecular formula can have different structural formulas called constitutional isomers, and the phenomenon is known as constitutional isomerism. Alkanes with four or more carbons showing multiple structures with the same molecular formula thereby exhibit constitutional isomerism.
The linear isomer of an alkane is prefixed by the term “n”; hence a linear isomer of pentane is known as n-pentane. Based on the type of branching, some of the...
The linear isomer of an alkane is prefixed by the term “n”; hence a linear isomer of pentane is known as n-pentane. Based on the type of branching, some of the...
18.2K
![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)

