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Updated: Jul 7, 2025

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在Saccharomyces cerevisiae mtDNA中的自发突变增加了mtDNA数量的细胞间变异

Elena Yu Potapenko¹, Nataliia D Kashko¹, Dmitry A Knorre²

¹Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 119991, Russia.

International journal of molecular sciences

|December 23, 2023

概括

mtDNA删除破坏了复制数的调节,增加了细胞间的变异. 全长线粒体DNA (mtDNA) 对于在细胞内保持一致的mtDNA水平至关重要.

科学领域:

细胞生物学细胞生物学
遗传学遗传学是一个
分子生物学分子生物学

背景情况:

细胞保持了线粒体DNA (mtDNA) 与核DNA (nDNA) 的特定比例,这表明了负反机制.
有限的实验数据存在关于mtDNA复制数的调节和潜在的干扰.
众所周知,mtDNA删除会增加mtDNA的丰富性,可能会干扰调节机制.

研究的目的:

调查mtDNA删除是否会破坏调节mtDNA拷贝数的负反循环.
为了确定mtDNA删除是否会增加mtDNA拷贝数的细胞对细胞差异.
探索全长mtDNA在调节mtDNA拷贝数恒温中的作用.

主要方法:

产生具有大量mtDNA删除的*Saccharomyces cerevisiae rho*菌株和具有耗尽mtDNA的*rho*菌株.
在单个酵母细胞中量化mtDNA,使用流细胞计和DNA间隙染料SYTOX绿色.
分析不同细胞周期阶段 (G1和G2/M) 的总DNA含量和细胞间异质性.

主要成果:

*rho*突变显著增加了G1和G2/M酵母细胞中总DNA含量的水平和细胞间异质性.
观察到的DNA含量异质性的增加与细胞大小无关.
在*rho*和*rho*两种菌株中mtDNA的耗尽导致SYTOX绿信号变异的显著下降.

结论:

mtDNA删除破坏了mtDNA复制数的调节,导致异质性增加.
全长 mtDNA 对于负反机制至关重要,这种反机制维持 mtDNA 复制量恒常.
*rho* mtDNAs 部分逃脱了这种调节,导致 mtDNA 水平的观察变化.

关键词:

细胞循环中的细胞循环.细胞对细胞异质性的异质性删除删除删除删除删除遗传漂移是一种遗传漂移.我们的mtDNA mtDNA 酵母酵母是一种酵母.

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