通过PRAF2守门员控制CCR5细胞表面准
Elisa Da Silva1, Mark G H Scott1, Hervé Enslen1
1CNRS, INSERM, Institut Cochin, Université Paris Cité, F-75014 Paris, France.
International journal of molecular sciences
|December 23, 2023
概括
PRAF2作为一个守门员,在ER中保留化学因受体CCR5. 这种蛋白质-G蛋白结合受体相互作用阻止了CCR5向细胞表面的输出,反对护送蛋白CD4的作用.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细胞表面蛋白质的运输依赖于ER出口,由护卫和守门者蛋白调节.
- G蛋白结合受体 (GPCR) 对于细胞信号传递和药物向至关重要.
- 已知PRAF2在ER中保留GABAB受体子单元.
研究的目的:
- 调查PRAF2在化学因子受体CCR5.5的ER出口中的作用.
- 阐明PRAF2和CCR5.5之间的相互作用机制.
- 为了比较PRAF2和CD4在CCR5贩运中的功能.
主要方法:
- 生物发光共振能量转移 (BRET) 用于亚细胞定位和近距离测试.
- 同免疫沉 (Co-IP) 证实蛋白相互作用.
- 位点定向突变发生,以研究CCR5.5内的特定动机.
主要成果:
- PRAF2以度依赖的方式抑制CCR5向血输出.
- PRAF2与CCR5的跨膜域相互作用,而不是其C端尾部.
- CCR5的潜在保留动机突变会影响CD4相互作用,但不会影响PRAF2结合.
- CD4促进了CCR5的出口,与PRAF2形成对抗作用.
结论:
- PRAF2作为CCR5的守门员,防止其ER退出.
- PRAF2和CCR5之间的相互作用涉及跨膜域.
- PRAF2和CD4在调节CCR5流入细胞表面的过程中具有相反的作用,可能是在COPII囊泡招募阶段.
关键词:
布雷特·布雷特是什么意思埃雷斯-埃雷斯是什么意思G蛋白结合受体的受体是G蛋白结合受体的受体.生物传感器生物传感器贩卖细胞,贩卖细胞.细胞内膜网膜的内oplasmic网膜.护航蛋白质护航蛋白质是什么守门人 守门人 守门人 守门人更多相关视频
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