尿液沉积细胞中的急性损伤和BK多瘤病毒
Sahra Pajenda1, Daniela Anna Gerges1, Raimundo Freire2,3,4
1Department of Internal Medicine III, Division of Nephrology and Dialysis, Medical University of Vienna, 1090 Vienna, Austria.
International journal of molecular sciences
|December 23, 2023
概括
在急性损伤 (AKI) 期间,可以在尿沉积细胞中检测到BK多聚马病毒 (BKV) 复制的非侵入性方法. 这种方法有助于识别患有BK病毒相关脏病 (BKPyVAN) 和病毒病的脏移植接受者.
科学领域:
- 病毒学 病毒学
- 腎臟病學 (nephrology) 是一種醫學.
- 免疫学 免疫学 免疫学
背景情况:
- 乙基多病毒 (BKV) 常见于免疫抑制的个体,特别是移植接受者.
- BKV感染可能导致BK病毒相关性病 (BKPyVAN) 和出血性囊炎.
- 目前的诊断包括PCR,免疫组织化学和诱细胞检测,但需要使用非侵入性方法来评估BKV复制.
研究的目的:
- 评估一种非侵入性方法,用于检测急性损伤 (AKI) 患者在尿液沉积细胞中的BK多病毒复制.
- 评估这种方法在识别移植接受者 (KTX) 患有BKV相关并发症风险时的有用性.
- 为了比较BKV检测在KTX和非移植 (非KTX) 患者与AKI.
主要方法:
- 用RNA提取,逆转录和病毒特异性qPCR检测79名患有AKI的患者尿液沉积细胞中的BKV复制.
- 针对VP1 BKV蛋白的免疫光染色使用共聚焦显微镜进行.
- 患者根据移植史 (KTX与非KTX) 和AKI阶段进行了分类.
主要成果:
- 在22名患有AKI (11个KTX,11个非KTX) 的患者的尿液沉积细胞中检测到BK病毒复制.
- 在AKI III期的患者中,BKV副本的发生频率更高.
- 在KTX患者中观察到更高的BKV拷贝数,这些患者有BKPyVAN的病史或目前的BKPyVAN. 只有在KTX患者中发现了BKPyVAN的诱细胞.
结论:
- 在AKI期间,可以在尿液沉积细胞中非侵入性检测BKV复制.
- 这种方法可以识别KTX患者有风险的病毒病和BKPyVAN.
- 这种方法可以作为一种有价值的查工具,用于监测和指导KTX患者经历AKI的免疫抑制.
相关概念视频
Nephrons
2.5K
The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
2.5K
Renal Corpuscle
2.3K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
2.3K
Kidney Structure
69.8K
The kidneys are two large bean-shaped organs located in the upper abdomen. They filter the blood several times a day to remove toxins and rebalance water and electrolytes of the circulatory system via the renal veins. The kidneys receive blood directly from the heart via the renal arteries. These arteries enter the kidney at the hilum, the concave surface of the bean, where they branch and divide into smaller vessels and capillaries.
69.8K
Internal Anatomy of the Kidney
1.8K
The kidneys are essential organs in the human body, performing a myriad of tasks that maintain homeostasis and overall health.
Anatomical Position and Dimensions
The kidneys are retroperitoneal organs positioned against the posterior abdominal wall on either side of the spine, roughly between the twelfth thoracic and third lumbar vertebrae. Each kidney is typically 10-12 cm long, 5-6 cm wide, and 3-4 cm thick, weighing about 150 grams.
Renal Cortex
The outermost region of the kidney is the...
Anatomical Position and Dimensions
The kidneys are retroperitoneal organs positioned against the posterior abdominal wall on either side of the spine, roughly between the twelfth thoracic and third lumbar vertebrae. Each kidney is typically 10-12 cm long, 5-6 cm wide, and 3-4 cm thick, weighing about 150 grams.
Renal Cortex
The outermost region of the kidney is the...
1.8K
Filtration and Urine Formation
50.1K
The function of the kidneys is to filter, reabsorb, secrete, and excrete. Every day the kidneys filter nearly 180 liters of blood, initially removing water and solutes but ultimately returning nearly all filtrates into circulation with the help of osmoregulatory hormones. This process removes wastes and toxins but is also crucial to maintain water and electrolyte levels. Most of these functions are performed by the tiny but numerous nephrons contained within the kidneys.
50.1K


