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对电和溶剂造PCL-PLGA的研究混合了嵌入诱导多能干细胞的支架,用于组织工程

Mariella Rosalia¹, Martina Giacomini¹, Erika Maria Tottoli¹

¹Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.

|December 23, 2023

概括

来自聚乳糖合糖酸 (PLGA) 和聚烯酸 (PCL) 混合物的组织工程支架是使用电 (ES) 和溶剂造 (SC) 创建的. SC支架显示出更好的细胞活力,而这两种方法都保持了诱导多能干细胞 (iPSCs) 的多能性.

科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
聚合物科学聚合物科学

背景情况:

开发用于组织工程的先进生物材料对于再生医学至关重要.
聚乳糖酸 (PLGA) 和聚烯酸 (PCL) 是广泛使用的可生物降解聚合物.
研究不同的制造方法会影响脚手架的性能和生物性能.

研究的目的:

使用电 (ES) 和溶剂造 (SC) 设计,生产和表征PLGA/PCL支架.
评估聚合物混合物成分对脚手架物理化学性质的影响.
评估这些支架对于培养诱导多能干细胞 (iPSCs) 的适用性.

主要方法:

通过电 (ES) 和溶剂造 (SC) 制造PLGA/PCL脚手架.
物理化学表征包括纤维直径,扬模量 (YM),接触角度和吸水量.
使用XTT试验和基因表达分析 (RT-PCR,RT-qPCR) 评估支架适合用于iPSC培养.

主要成果:

通过控制PCL度,ES支架允许量身定制纤维直径 (0.6-1.0μm) 和YM (9-22 MPa).
与ES脚手架 (110°) 相比,SC脚手架具有"泡状"的地形,接触角度较低 (74°).
由于可湿性和YM,SC支架在5天内显示出更高的细胞活力,而多能性维护在两种方法中都类似.

结论:

无论是ES还是SC的制造方法都会产生具有独特特性的PLGA/PCL支架.
由于增强的表面特性,SC脚手架显示出支持iPSC可行性的前景.
进一步的研究可以为特定的组织工程应用优化这些支架,保持干细胞多能性.

关键词:

电力旋转是指电力旋转.这就是 iPSC 的意义.聚合物混合物的聚合物混合物.溶剂造/颗粒物浸出溶剂造

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