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在重症急性呼吸综合征相关的冠状病毒2和DENV之间绘制IgA位和交叉反应

Salvatore G De-Simone^1,2,3,4, Paloma Napoleão-Pêgo^1,2, Guilherme C Lechuga^1,2

¹Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, RJ, Brazil.

|December 23, 2023

概括

这项研究绘制了COVID-19 S蛋白上的IgA表位图,揭示了与其他病毒的交叉反应性. 鉴定的表位体可以帮助监测粘膜免疫力和开发COVID-19的新诊断工具.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
生物化学生物化学

背景情况:

COVID-19疫苗可以降低严重程度,但不能降低感染/传播.
目前的抗体测试 (IgM,IgG) 不评估粘膜免疫力.
需要进行测试来测量粘膜体对SARS-CoV-2的免疫反应.

研究的目的:

为了全面地绘制SARS-CoV-2 S蛋白的IgA表位.
研究这些表位体与其他病原体的交叉反应性.
为IgA检测开发一种敏感和特定的ELISA-测定.

主要方法:

使用SPOT合成和患者血清进行IgA表位图绘制.
对表观物种进化趋势的生物信息分析.
化学合成和ELISA验证所选的表位物.

主要成果:

在S1和S2子单元上确定了40个IgA表位.
与登革热病毒 (DENV) 和其他冠状病毒观察到显著的交叉反应性.
经验证的ELISA-测定显示了高灵敏度 (90-99.96%) 和特异性 (100%).

结论:

揭示了S蛋白的IgA表位,突出了交叉反应性.
在SARS-CoV-2变种中存在保存的IgA表位,只有一个例外.
交叉反应性表明IgA诊断中全S蛋白/RBD的局限性;特定的表位提供了监测粘膜免疫和疫苗开发的潜力.

关键词:

在 COVID-19 疫情中,在IgA表位上.在IgA-诊断方面.这就是SARS-CoV-2病毒.在SARS-CoV-2变种中.交叉反应的表观物.粘膜免疫力粘膜免疫力

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