考克萨基病毒A14的分子流行病学和演变
Liheng Yu1, Qin Guo1, Haiyan Wei2
1National Polio Laboratory, WHO WPRO Regional Polio Reference Laboratory, National Health Commission Key Laboratory for Biosecurity, National Health Commission Key Laboratory of Medical Virology, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Viruses
|December 23, 2023
概括
这项研究分析了Coxsackievirus A14 (CVA14) 的演变,揭示了它的基因型,起源于1942年,以及复杂的迁移模式. 这些发现填补了了解CVA14流行病学及其在手足口腔疾病中的作用的差距.
科学领域:
- 病毒学 病毒学
- 分子流行病学分子流行病学
- 植物动力学是关于植物动力学的.
背景情况:
- 在手足口病 (HFMD) 中,非肠道病毒71和非萨基病毒A16的全球流行率不断增加,需要对其他肠道病毒进行研究.
- 考克萨基病毒A14 (CVA14),是肠道病毒A物种的成员,在全球范围内流行,但它的进化动态和重组模式仍然不太清楚.
- 对于CVA14存在有限的分子流行病学数据,这阻碍了对其全球传播和遗传多样性的全面理解.
研究的目的:
- 为了研究Coxsackievirus A14 (CVA14) 菌株的分子流行病学特征.
- 确定CVA14.的进化动态,遗传多样性和重组模式.
- 为了确定植物地理,并估计CVA14的起源.
主要方法:
- 从中国大陆 (2009-2019) 的HFMD患者中分离并对15种CVA14菌株进行完整基因组测序.
- 从孤立菌株和GenBank数据中对完整基因组序列和全长VP1编码区域的生物信息分析.
- 植物动力学和植物地理学分析,包括贝叶斯天际线图和重组检测.
主要成果:
- 根据VP1序列分歧 (>15%) 将CVA14菌株分为七种基因型 (A-G).
- 植物动力学分析估计平均替代率为5.35 × 10^-3替代/地点/年,最近的共同祖先 (tMRCA) 可以追溯到1942年 (1930-1950年).
- 贝叶斯天际线图显示了自2004年以来的人口规模波动,全球和中国境内的迁移路线已被确定. 观察到四种重组模式,其中CVA2,CVA4,CVA6,CVA8和CVA12可能充当重组供体.
结论:
- 这项研究提供了CVA14的第一个全面的分子流行病学分析,填补了关键的知识空白.
- 这些发现丰富了全球CVA14序列数据库,并为未来对其全球流行病学研究奠定了基础.
- 了解CVA14的遗传多样性,进化史和再组合对于管理HFMD疫情至关重要.
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