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Xingpo Liu¹, Dawei Yan¹, Shan Peng²

¹Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.

|December 23, 2023

概括

坦布苏病毒 (TMUV) 对哺乳动物构成威胁. 一种高度毒性的HB菌株通过嗅觉通路侵入小鼠大脑,引起严重的炎症并突出显示潜在的风险.

科学领域:

病毒学病毒学
病变的发生和发病.
哺乳动物感染感染

背景情况:

坦布苏病毒 (TMUV) 疫情在家禽中造成重大经济损失.
TMUV对哺乳动物的潜在威胁仍未得到充分研究.

研究的目的:

研究 TMUV HB 菌株在哺乳动物中的毒性和神经侵入性潜力.
阐明小鼠中枢神经系统中TMUV HB病原体的分子机制.

主要方法:

用TMUV HB菌株和参考菌株对BALB/c小鼠进行鼻腔注射.
病毒蛋白 (E和NS3) 的遗传分析.
在脑组织中测量炎症性细胞因子 (IL-1β,IL-6,IL-8,IFN-α/β) 和通路 (RIG-I,IRF7) 的表达.

主要成果:

TMUV HB在小鼠中表现出高毒性和神经侵入性,利用嗅觉上皮的通路.
在E和NS3蛋白中的特定突变 (K326E) 影响了神经病毒发作.
与突变病毒相比,TMUV HB诱导的炎症反应显著更高,并上调RIG-I和IRF7.
在外源性干扰素-β (IFN-β) 应用时观察到恶化的疾病,这表明过度刺激的IFN反应具有有害影响.

结论:

由于其神经侵入性和毒性特性,TMUV HB菌株对哺乳动物构成潜在的风险.
替代K326E影响TMUV神经病毒性,而HB菌株通过RIG-I-IRF7通路触发强烈的IFN反应.
过度刺激大脑的IFN反应在TMUV感染期间可能是有害的.

关键词:

在 RIG-I-IRF7F7 中,坦布苏病毒 (Tembusu virus) 是一种病毒.包裹蛋白质是一种包裹蛋白质.这是一种炎症性炎症.突变是一种突变.

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