猪流行性腹病毒从韩国现场样本的分子表征
Bac Tran Le1, Hansani Chathurika Gallage1, Min-Hui Kim1
1College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.
Viruses
|December 23, 2023
概括
韩国的猪流行性腹病毒 (PEDV) 菌株显示出遗传多样性和进化. 尖端蛋白中的关键突变可能会影响病毒的致病性和免疫逃避,突出显示需要持续监测.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 猪健康 猪健康
背景情况:
- 猪流行性腹病毒 (PEDV) 是一个重要的肠道病原体,在全球猪业中造成了巨大的经济损失.
- 自1992年出现以来,PEDV一直对养猪业构成持续的挑战,需要持续监测其遗传演变.
- 了解循环PEDV菌株的分子特征对于开发有效的控制策略至关重要.
研究的目的:
- 研究来自韩国的猪流行性腹病毒 (PEDV) 实地分离物的遗传多样性和分子特征.
- 对尖 (S) 糖蛋白基因进行基因分析,以了解这些PEDV菌株的进化关系.
- 为了确定特定的氨基酸替代和改变可能影响病毒性质的糖化模式.
主要方法:
- 从六个PEDV实地样本中分离和测序完整的尖峰 (S) 糖蛋白基因.
- 用S基因序列进行遗传学分析,以确定遗传集群和关系.
- 生物信息学分析包括BLAST搜索序列标识和氨基酸替代物和N相关的糖化变化的识别.
主要成果:
- 六个PEDV分离物被识别并聚集在G2b子组内,表现出高核酸 (97.9-100%) 和氨基酸 (97.4-100%) 的同一性.
- 特定的氨基酸替代物,包括关键表位的新变化 (562S→F和763P→L),在一个分离物 (CNU-22S11) 中被确定.
- 在CNU-22S11分离物中观察到N-链接的糖化位点的变化,这表明病毒适应正在进行中.
结论:
- 在韩国养猪场中,PEDV的多种和不断发展的菌株普遍存在.
- 在尖端蛋白中识别的突变可能会影响病毒受体结合,致病性和宿主免疫系统逃避.
- 这项研究增强了对韩国PEDV流行病学的理解,为未来的控制和预防工作提供了信息.
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