相关实验视频
Updated: Jul 7, 2025

12:04
FtsZ Polymerization Assays: Simple Protocols and Considerations
Published on: November 16, 2013
15.3K
来自人类致病细菌Helicobacter pylori的FtsZ异常弱且延迟的GTPase活性
Sumiran Kumar Gurung1,2, Sangeeta3, Priyanka Dubey1
1Special Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi India.
Journal of biochemistry
|December 23, 2023
概括
杆菌FtsZ (HpFtsZ) 是一种pH敏感的GTPase,受酸性抑制,与大肠杆菌FtsZ不同. 这种低效率可能解释了H. pylori的缓慢生长.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 结构生物学 结构生物学
背景情况:
- FtsZ蛋白对于细菌细胞分裂至关重要,为分裂组分形成支架.
- 人们对Helicobacter pylori FtsZ (HpFtsZ) 的了解很少,尤其是它在酸性环境中的功能.
- 杆菌是I类致癌物,在酸性环境中繁荣发展.
研究的目的:
- 为了研究HpFtsZ.的生物化学特性.
- 了解pH,和时间对HpFtsZ活动的影响.
- 为了比较HpFtsZ与特征很好的大肠杆菌FtsZ (EcFtsZ).
主要方法:
- 在不同的pH值和离子度下测定GTPase活性.
- 在HpFtsZ和EcFtsZ之间比较核酸水解速率.
- 分子动力学模拟分析FtsZ结构动力学和GTP结合.
主要成果:
- 在酸性条件下,HPFtsZ表现出pH依赖的GTPase活性,被抑制.
- 离子对HPFtsZGTPase活性至关重要,但高度具有抑制作用.
- 与EcFtsZ相比,HpFtsZ的核酸水解显著较低和较慢.
- 分子动力学揭示了GTP结合变异键网络,减少结合裂体积并释放GTP.
- GTPase活性与结合裂体积减少相关,得到结合自由能分析的支持.
结论:
- HpFtsZ是一种pH敏感,低效的GTPase.
- 观察到的HpFtsZ的特性可能有助于Helicobacter pylori的缓慢生长率.
相关概念视频
Cytoskeletal Proteins in Bacteria
3.4K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.4K
Actin Filament Depolymerization
3.1K
Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
In F-actin, the ADF/cofilin proteins...
3.1K
Destabilization of Microtubules
2.7K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.7K
GTPases and their Regulation
8.4K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
Large G-proteins,...
8.4K
Mechanism of Filopodia Formation
2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
Small GTPases - Ras and Rho
4.0K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
4.0K

