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Updated: Jul 7, 2025

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

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在异质细胞群中检测定义的T细胞克隆的协议

Andrea Gobbini1, Alessandra Bandera2, Renata Grifantini3

  • 1INGM, Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", 20122 Milan, Italy.

STAR protocols
|December 23, 2023
PubMed
概括

这项研究引入了一项新协议,用于在复杂细胞混合物中识别特定的T细胞克隆. 该方法使用T细胞受体测序来检测预定义的克隆,有助于表型和功能分析.

关键词:
生物信息学是一种生物信息学.细胞隔离细胞的隔离.流动细胞计量 (Flow Cytometry) 是一种流动细胞计.基因表达 基因表达免疫学 免疫学 免疫学分子生物学分子生物学序列分析是指进行序列分析.一个单细胞单细胞.

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T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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