全长的转录组揭示了Oratosquilla oratoria的循环偏光反应相关的分子遗传特征
Fangrui Lou1, Zhongjie Ren1, Yongzheng Tang1
1School of Ocean, Yantai University, Yantai 264003, Shandong, China.
Comparative biochemistry and physiology. Part D, Genomics & proteomics
|December 23, 2023
概括
蒙蒂斯独特地检测到循环偏振光 (CPL). 这项研究揭示了它们眼睛中的关键基因和替代拼接,提高了我们对它们复杂的视觉和CPL识别机制的理解.
科学领域:
- 海洋生物学 海洋生物学
- 视觉科学 视觉科学 视觉科学
- 基因组学就是基因组学.
背景情况:
- 蒙蒂斯拥有感知循环偏光 (CPL) 的独特能力,这是其他动物没有观察到的特征.
- 这种非凡的视觉能力的分子和遗传基础在很大程度上仍未被探索.
研究的目的:
- 为了研究神灵Oratosquilla oratoria中CPL识别的分子遗传基础.
- 识别与CPL感知相关的积极选择下的基因,并分析复合眼中的替代拼接事件.
主要方法:
- 在Oratosquilla oratoria的多个组织中进行全长 (FL) 转录组测序.
- 进行比较的转录组学来检测由CPL选择的基因.
- 在各种光条件下对复合眼的FL转录组序列测定,以确定替代拼接 (AS).
主要成果:
- 假设GNβ基因是积极选择的,这表明它在CPL信号转导中的作用.
- 与CPL识别相关的替代拼接事件在眼睛转录的LWS,ARR和TRPC区域中被确定.
- 确定了来自LWS,UV和Peropsin基因家族的51个序列,显示了opsins特征的保存结构和图案特征.
结论:
- 这些发现提供了对灵长中CPL识别背后的遗传机制的重要见解.
- 鉴定的基因和替代拼接模式有助于对Oratosquilla oratoria和相关物种的视觉理论的精细理解.
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