伊弗梅克丁通过β-tubulin的预测结合部位影响Arabidopsis thaliana的微管
Yevhen Kustovskiy1, Pavel Karpov2, Yaroslav Blume2
1Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Baidy-Vyshnevetskoho str., 2a, Kyiv, 04123, Ukraine; National University of Kyiv-Mohyla Academy, Skovorody str., 2, Kyiv, 04070, Ukraine.
Plant physiology and biochemistry : PPB
|December 23, 2023
概括
伊弗梅克会破坏植物细胞的微管,影响根的生长. 分子模拟证实了它与β-tubulin的结合,稳定了微管,并防止了脱聚合.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 毒理学 毒理学 毒理学
背景情况:
- 作为一种强有力的杀虫剂的伊弗梅克显示了吸收和对植物生理学的负面影响.
- 了解ivermectin的植物分子标是非常重要的,因为它对非标生物有毒性.
研究的目的:
- 为了研究ivermectin对Arabidopsis thaliana细胞中的微管的作用.
- 阐明ivermectin在植物细胞中的作用的分子机制.
主要方法:
- 用ivermectin治疗的Arabidopsis thaliana根细胞的显微镜分析.
- 来自A. thaliana和Haemonchus contortus的β-tubulin复合的ivermectin的分子动力学模拟.
主要成果:
- 伊弗梅克丁 (250μg mL-1) 破坏了A. thaliana根细胞中的微管网,导致改变方向,收缩和捆绑.
- 分子动力学模拟证实了ivermectin与β-tubulin分类基位结合.
- 艾弗梅克丁结合稳定了M循环并增强了侧面接触,防止了微管的脱聚合.
结论:
- 甲胺直接准并破坏植物细胞微管,影响细胞结构和潜在的生长.
- 这项研究为ivermectin的植物毒性提供了分子洞察力,将β-tubulin确定为关键目标.
- 这些发现有助于了解ivermectin对非目标植物生物的生态风险.
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