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PeFtsH5 负面调节了 Phalaenopsis equestris 线粒体中的生物应激反应

Yiding Wu¹, Yang Meng¹, Long Zhang²

¹Development Centre of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China; Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.

Journal of plant physiology

|December 23, 2023

概括

降低Phalaenopsis equestris中PeFtsH5基因的调节可以增强植物对疾病的抵抗力,但也可以加速细胞亡并增加ROS. 这种线粒体蛋白酶作为同聚合物起作用,影响细胞命运和应激反应.

科学领域:

线粒体生物学线粒体生物学
植物病理学植物病理学
分子遗传学分子遗传学

背景情况:

线粒体平衡对于细胞命运至关重要,影响细胞亡和自.
依赖ATP和Zn2+的FtsH蛋白酶是维护线粒体平衡的关键.

研究的目的:

研究PeFtsH5基因及其编码的线粒体蛋白酶在Phalaenopsis equestris中的作用.
了解PeFtsH5在植物应激反应和线粒体平衡中的作用.

主要方法:

在Nicotiana benthamiana和P. equestris中对PeFtsH5同源的基因表达分析.
评估与SA合成和防御反应相关的抗病性,亡,活性氧物种 (ROS) 水平和基因表达.
双膜实验以确定PeFtsH5蛋白质的聚合.

主要成果:

降低PeFtsH5的调节增加了SA合成和防御反应基因,增强了生物应激抵抗力,但导致细胞损伤和加速亡.
PeFtsH5蛋白形成独有的同聚合物,并且不会与其他线粒体FtsH蛋白酶相互作用.
在PeFtsH5下调后观察到ROS水平的增加.

结论:

PeFtsH5在调节植物对生物压力的反应和维护线粒体平衡中发挥着重要作用.
在P.equestris细胞中,PeFtsH5蛋白酶作为同聚合物起作用.
这些发现为开发抗压力Phalaenopsis equestris品种提供了洞察力.

关键词:

细胞亡是细胞的亡.在FtsH5中使用.线粒体中的线粒体.类 (Phalaenopsis equestris) 是一种类植物.病毒诱导的基因沉默 (VIGS)

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