通过抗超耐药菌株Achromobacter sp.的抗沉和去除. 25M- 25M的使用方法
Prakash C Loni1, Weiqi Wang2, Xuan Qiu2
1State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430078, China; School of Environmental Studies, China University of Geosciences, Wuhan, 430078, China; Department of Earth Sciences, National Cheng Kung University, Tainan, 701, Taiwan.
Environmental research
|December 23, 2023
概括
本土的细菌,阿克罗巴克特 sp. 25-M,有效地通过沉Sb (III) 和Sb (V) 到矿物质中去除溶解的反 (Sb). 细菌产生的外聚合物物质 (EPS) 在这种反生物修复过程中发挥着关键作用.
科学领域:
- 环境微生物学 环境微生物学
- 地质化学 地质化学
- 生物地质化学生物地质化学
背景情况:
- 确立了微生物对抗氧生物地质化学的影响.
- 来自活跃矿山的土著细菌在溶解的行为中的特定作用仍然不清楚.
研究的目的:
- 为了评估Achromobacter sp.的容量. 在沉和去除溶解的抗物种 (Sb (V) 和Sb (III)) 时, 25-M是一种来自西冈山抗矿藏的抗耐药细菌.
- 调查细菌外聚合物物质 (EPS) 在抗相互作用和去除中的作用.
主要方法:
- 对抗氧耐药细菌Achromobacter sp.的分离和表征 25万美元,25万美元.
- 在存在或缺少25-M时,使用Sb (III) 和Sb (V) 的化实验.
- 使用X射线衍射 (XRD) 和能量分散光谱 (EDS) 分析沉物.
- 使用扫描电子显微镜 (SEM) 的形态分析和使用福里埃变换红外 (FT-IR) 光谱仪对EPS的功能组分析.
主要成果:
- 亚克罗巴克特菌 sp. 的存在. 25-M表现出高的Sb (III) 耐性,但没有氧化Sb (III).
- 在的存在下,细菌产生了显著的外聚合物物质 (EPS).
- 96小时后,88%的Sb (V) 和80%的Sb (III) 在25-M系统中被清除.
- 在微生物系统中,XRD和EDS证实了从Sb (III) 中形成的瓦伦丁酸盐 (Sb2O3) 和从Sb (V) 中形成的莫邦酸盐 (NaSb (OH) 6),在无生物对照中没有沉物.
- FT-IR分析表明,EPS (-OH, -COO, C-H, N-H) 内的功能组促进了与抗氧化物种的复合,配体交换和化,促进了矿物沉.
结论:
- 外聚合物物质 (EPS) 在抗生物沉中发挥着根本性的作用,由Achromobacter sp. 25万美元,25万美元.
- 在EPS功能组和抗氧物种之间的相互作用驱动特定抗氧矿物质的形成.
- 这项研究提供了一种通过微生物EPS介导沉来进行抗生物修复的新策略.
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