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从临床益处的角度来看,甲基因组下一代测序测试

Mengyuan Chen¹, Yingying Cai², Lisha Wang³

¹Department of Laboratory Medicine, Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University, China; Department of Laboratory Medicine, Enze Hospital, Taizhou Enze Medical Center (Group), China; Key Laboratory of System Medicine and Precision Diagnosis and Treatment of Taizhou, China.

Clinica chimica acta; international journal of clinical chemistry

|December 23, 2023

概括

甲基因组下一代测序 (mNGS) 显示了对传染病的临床益处,但最佳策略取决于患者的选择,样本类型和时间. 定制应用程序增强了诊断价值.

科学领域:

临床微生物学临床微生物学
基因组医学是一种基因组医学.
传染病诊断传染病诊断传染病诊断

背景情况:

下一代元基因组测序 (mNGS) 提供了快速的病原体诊断.
对mNGS的临床应用策略需要进一步研究,以最大限度地提高效益.

研究的目的:

评估mNGS在各种环境中的诊断性能和临床影响.
确定影响传染病诊断中mNGS实用性的因素.

主要方法:

追溯分析775个来自疑似传染病患者的样本.
基于最终诊断的诊断性能,临床相关性和影响的评估.
探索影响因素,包括样本类型和干预时间.

主要成果:

84.26%的mNGS测试具有临床相关性,样本类型是关键因素.
40.77%的测试具有积极的临床影响;在特定的患者群体和样本类型 (BALF,CSF) 中观察到更高的影响.
及时采样和报告 (例如,在2天内) 改善了临床效益率.

结论:

在不同的临床环境中,mNGS的临床益处在很大程度上有所不同.
优化患者选择,样本选择,抽样时间和报告时间对于提高mNGS实用性至关重要.
需要一个合理的战略来促进mNGS的科学应用和临床价值.

关键词:

成人传染病是成年人的传染病.应用策略应用策略临床影响临床影响临床相关性临床相关性在mNGS测试中进行测试.

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