利用咖啡废弃物作为可持续原料,通过微生物发酵来生产高产乳酸
Seulbi Kim1, Jong-Cheol Kim2, Yeong Yeol Kim3
1Technology Innovation Research Division, World Institute of Kimchi, Gwangju, Republic of Korea; Department of Agricultural Chemistry, Chonnam National University, Gwangju 61186, Republic of Korea.
The Science of the total environment
|December 23, 2023
概括
通过优化预处理和发酵过程,咖啡废料可以有效地转化为乳酸. 这种可持续的方法为工业乳酸生产提供了具有成本效益的替代方案.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 可持续化学 可持续化学
背景情况:
- 高的基质成本阻碍了微生物发酵以生产乳酸.
- 咖啡废料提供了一个可持续的,低成本的原料替代品.
- 优化咖啡废物利用对于高效的乳酸合成至关重要.
研究的目的:
- 调查使用咖啡废弃物作为乳酸生产原料的可行性.
- 优化预处理方法和酶组合,以提高咖啡废物中糖分的释放.
- 为了评估乳酸发酵的效率,使用优化的咖啡废物水解剂.
主要方法:
- 咖啡废物的预处理,使用过氧化和酸.
- 酶性水解使用了Viscozyme L,Celluclast 1.5 L和Pectinex Ultra SP-L的组合.
- 乳酸发酵由 Lactiplantibacillus plantarum 进行的 WiKim0126.6. 乳酸发酵
主要成果:
- 在4%的基质度下,从咖啡废物中实现了78.9%的糖转化率.
- 生产了22.8g/L的乳酸,相当于理论最大产量的99.6%.
- 在24小时内获得0.95g/L/h的乳酸生产率.
结论:
- 咖啡废料是可持续乳酸生产的可行和环保原料.
- 优化的预处理和酶性水解显著改善了糖的释放.
- 有效的乳酸发酵可以从咖啡废弃物水解剂中实现.
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