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探索非向分析在产前暴露组的表征中的应用

Garret D Bland¹, Dimitri Abrahamsson¹, Miaomiao Wang²

¹Department of Obstetrics, Gynecology and Reproductive Sciences, Program on Reproductive Health and the Environment, University of California San Francisco, San Francisco, CA, United States.

The Science of the total environment

|December 23, 2023

概括

这项研究优化了非向分析 (NTA) 以发现母亲和婴儿血液中以前未报告的化学物质,这对于了解产前化学物质暴露和长期健康影响至关重要.

科学领域:

环境化学环境化学
毒理学毒理学毒理学
分析化学分析化学

背景情况:

了解子宫内化学物质暴露对母婴健康至关重要.
非定向分析 (NTA) 是识别未知的化学物质的强大工具.

研究的目的:

调整和评估NTA管道中的方法,以提高在母婴血清中的化学发现.
在产前暴露中识别鲜为人知的或以前未报告的化学物质.

主要方法:

在母体和带血清样本上利用液体染色学四极飞行时间质谱法 (LC-QTOF/MS).
开发并比较了两个具有不同检测频率截止值的NTA数据处理管道.
使用EPA CompTox,HMDB,MS/MS光谱匹配和内部算法进行注释的化学特征.

主要成果:

在超过32,000个检测到的化学特征中,大约有72%的特征被注释出来.
通过分析标准确认了22种化合物,并使用MS/MS.初步确定了88种化合物.
在人类血液中发现了36种以前未报告的化学物质,其中9种在不到五项研究中报告.

结论:

优化的NTA方法显著提高了人类血清中新型和未报告的化学物质的发现.
这种方法对于全面描述产前化学物质暴露及其潜在的健康影响至关重要.

关键词:

数据管道数据管道暴露组体是指暴露组体.高分辨率的质谱学高分辨率质谱学肯德里克大质量缺陷不针对性的分析.产前暴露在产前暴露.

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