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Updated: Jul 7, 2025

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

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肠道共生动物的随机编码的转子子突变库II:应用功能遗传学的库

Carlos Geert Pieter Voogdt1, Surya Tripathi2, Stefan Oliver Bassler3

  • 1Genome Biology Unit, EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany; Structural and Computational Biology Unit, EMBL, Heidelberg, Germany.

Cell reports
|December 24, 2023
PubMed
概括

基因组规模的转子子突变库正在彻底改变肠道微生物群研究. 这些工具使得成本效益高的健身测定成为可能,揭示了对人类健康和疾病至关重要的微生物功能.

关键词:
CP: 微生物学 微生物学化学基因组学 化学基因组学基因型-表型映射的映射我们的肠道微生物群.主体-微生物相互作用转位子的突变发生是转位子的突变发生.

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DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
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