相关实验视频

Updated: Jul 7, 2025

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Published on: August 9, 2024

"黑暗"CO2在酸盐发酵中的固定,可通过通过空心纤维膜碳化直接输送CO2通过空心纤维膜碳化实现

Amanda G Godar¹, Timothy Chase², Dalton Conway²

¹School of Life Sciences, Arizona State University, Tempe, AZ, USA.

Bioprocess and biosystems engineering

|December 24, 2023

概括

这项研究引入了空心纤维膜 (HFMs),用于在无氧酸盐发酵中输送二氧化碳 (CO2). 这种方法为二碳酸盐提供了一个可持续的替代品,可以获得高酸盐产量.

科学领域:

生物技术和代谢工程生物技术和代谢工程
生物处理工程和优化优化
碳捕获和利用是碳的捕获和利用.

背景情况:

无氧糖酸盐发酵利用二氧化碳通过三碳酸循环固定,以实现高度,高产量生产.
传统的二氧化碳供应依赖于二碳酸盐/碳酸盐,这在能源上昂贵,并降低了工艺的可持续性.
工业应用需要一种替代的,可持续的二氧化碳输送方法.

研究的目的:

开发和评估复合体空心纤维膜 (HFMs),以在无氧发酵中有效地传递CO2.
研究模块化二氧化碳输送速率对工程化大肠杆菌酸盐生产的影响.
与传统的盐添加相比,评估基于HFM的CO2供应的经济和环境可行性.

主要方法:

复合体空心纤维膜 (HFMs) 的制造.
无细胞CO2质量转移测量使用一种新的恒定pH方法.
用工程造型大肠杆菌进行发酵研究的HFM生物反应器的建造和运行.
分析酸盐标位,产量和二氧化碳输送参数 (光压,HFM表面积).

主要成果:

HFM CO2输送显示了光圈压力/HFM表面积和CO2流量/体积率之间的线性相关性.
使用基于HFM的CO2供应,工程化大肠杆菌生产了高达64.5g/L的糖酸盐,葡萄糖产量为0.68g/g.
HFM的性能接近于常规二碳酸盐/碳酸盐添加的性能,并显示了重复使用的潜力.

结论:

基于HFM的二氧化碳输送是一种可行和可持续的替代碳酸盐/碳酸盐,用于酸盐发酵.
这项技术可以调整为最佳的二氧化碳供应,实现高精度的生产指标.
HFM技术对其他暗 CO2 固定发酵具有前景,促进了碳捕获和利用.

关键词:

暗 CO2 固定方式膜生物反应器是一种膜生物反应器.膜炭化碳化的情况.酸盐发酵的发酵方式

更多相关视频

$Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems$ 14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Published on: December 12, 2015

相关概念视频

The Citric Acid Cycle

The Citric Acid Cycle

The citric acid cycle, also known as the Krebs cycle or TCA cycle, consists of several energy-generating reactions that yield one ATP molecule, three NADH molecules, one FADH2 molecule, and two CO2 molecules.

Fates of Pyruvate

Fates of Pyruvate

Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...

The Citric Acid Cycle: Overview

The Citric Acid Cycle: Overview

In aerobic organisms, the citric acid cycle is the second stage of cellular respiration wherein molecules derived from the breakdown of carbohydrates, proteins, and fats are oxidized into carbon dioxide and energy. This process is also known as the tricarboxylic acid (TCA) cycle as the first product of the cycle, citric acid, contains three carboxyl groups in its structure. Alternatively, this cycle is also referred to as the Krebs cycle, in honor of its discoverer Sir Hans Krebs.
The citric...

Products of the Citric Acid Cycle

Products of the Citric Acid Cycle

The cells of most organisms—including plants and animals—obtain usable energy through aerobic respiration, the oxygen-requiring version of cellular respiration. Aerobic respiration consists of four major stages: glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation. The third major stage, the citric acid cycle, is also known as the Krebs cycle or tricarboxylic acid (TCA) cycle.

The Citric Acid Cycle: Output

The Citric Acid Cycle: Output

The citric acid cycle is termed an amphibolic pathway as it operates both anabolically and catabolically. The cyclic reactions balance the flux of the substrates to provide an optimal concentration of NADH and ATP to the cell.
Regulation of Citric Acid Cycle
The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
The primary substrate of the TCA cycle—acetyl CoA—is...

Pyruvate Oxidation

Pyruvate Oxidation

After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...