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ERF5.1通过与Lycium中的CCD4.1相互作用来调节胡卜素积累

Jianhua Zhao¹, Yuhui Xu¹, Haoxia Li²

¹National Wolfberry Engineering Research Center/Wolfberry Science Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, 750002, China.

Horticulture research

|December 25, 2023

概括

研究人员发现,乙烯响应因子5.1 (ERF5.1) 通过与卡洛分离二氧化酶4.1 (CCD4.1) 相互作用来调节中的胡卜素水平,这为促进胡卜素积累提供了新的途径.

科学领域:

植物分子生物学植物分子生物学
生物化学生物化学
遗传学是一个遗传学.

背景情况:

胡卜素是重要的天然颜料,具有显著的人类健康益处.
胡卜素分裂二氧化酶4 (CCD4) 和乙烯反应因子 (ERF) 是已知的胡卜素代谢的调节者.
在*Lycium*物种中,CCD4和ERF在胡卜素调节中的特定作用仍然未被描述.

研究的目的:

为了研究 *Lycium barbarum* 胡卜素裂变二氧化酶 4.1 (*LbCCD4.1*) 在胡卜素代谢中的功能.
为了确定和描述 *Lycium barbarum* 乙烯反应因子5.1 (*LbERF5.1*) 在胡卜素生物合成中的作用.
为了阐明*LbERF5.1*和*LbCCD4.1*之间在*Lycium*中调节胡卜素积累的分子相互作用.

主要方法:

来自*Lycium*参考基因组的*LbCCD*基因的生物信息注释.
基因表达分析将LbCCD4.1与水果发育过程中的胡卜素含量相关联.
在 *Nicotiana benthamiana* (NQ) 中进行过度表达和病毒诱导的基因沉默 (VIGS) 实验,以评估基因功能.
酵母单杂交和双露西法酶记者测定以确认转录因子促进体相互作用.
转录组分析以确定对*LbERF5.1*和*LbCCD4.1*过度表达的反应中差异表达的基因.

主要成果:

*LbCCD4.1*表达与五个水果发育阶段的胡卜素代谢物水平有显著的相关性.
过度表达*LbCCD4.1*导致转基因NQ叶中的β-胡卜素和β-密素的水平降低.
过度表达*LbERF5.1*导致转基因NQ叶子中胡卜素,特别是黄蛋白和紫素的显著减少.
*LbERF5.1*直接与*LbCCD4.1*的促进体结合,增强其表达.
转录组分析显示,在*LbERF5.1*和*LbCCD4.1*过度表达的线条中,类似的全球表达模式影响了关键的胡卜素生物合成基因.

结论:

发现了一种涉及*LbERF5.1*和*LbCCD4.1*之间的相互作用的新型分子机制,用于*Lycium*中的卡洛类积累.
*LbERF5.1*作为*LbCCD4.1*表达的积极调节者,影响着胡卜素生物合成.
这种相互作用为基因工程策略提供了潜在的目标,以增强Lycium*中的胡卜素含量.

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