相关实验视频

Updated: Jul 7, 2025

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

Published on: May 6, 2022

细胞内膜网膜介质Drosophila翅膀的发展

Laura Faith George¹, Mikaela Lynn Follmer¹, Emily Fontenoy¹

¹Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.

|December 25, 2023

概括

内部调整 (Irk) 通道调节释放和骨形态遗传蛋白 (BMP/Dpp) 释放,以促进多虫翅膀的发育. 适当的ER平衡对于BMP/Dpp释放和翅膀模式至关重要.

科学领域:

发展生物学发展生物学
细胞信号传递细胞信号传递
离子通道生理学离子通道生理学

背景情况:

形态原体呈现的时间动态对于组织模式至关重要,但控制形态原体释放的机制仍然不清楚.
内部调整 (Irk) 通道影响Drosophila翅膀发育中的细胞内和骨形态遗传蛋白 (BMP/Dpp) 释放.
伊尔克通道调节各种细胞类型的信号传递,这表明它在分泌中起着保留的作用.

研究的目的:

调查内细胞网膜 (ER) 调节在BMP/Dpp释放和Drosophila翅膀发育中的作用.
测试电活动控制ER释放,影响BMP释放的假设.

主要方法:

使用RNA干扰 (RNAi) 来降低关键ER调节者的表达 (Stim,Orai,SERCA,SK,Best2).
实时成像追踪了Drosophila翅膀盘和幼翅膀中的和DPP释放.
免疫组织化学评估了SMAD酸化,这是BMP/Dpp信号的下游标志物.

主要成果:

降低了Stromal相互作用分子1 (Stim) 和SarcoEndoplasmic Reticulum ATPase (SERCA) 功能的作用,导致的短暂幅度和频率降低.
抑制Stim和SERCA导致BMP/Dpp释放减少.
这些变化与被破坏的翅膀形态相关.

结论:

对于调节BMP/Dpp释放而言,ER平衡至关重要.
适当的BMP/Dpp信号,由ER动态控制,对于Drosophila翅膀的发展至关重要.

关键词:

在BMP中,BMP是BMP.德洛索菲拉的翅膀的发展发展.这里是Orai Orai.这就是 SERCA SERCA.这就是Stim Stim Stim.贝斯特罗芬 (bestrophin) 是一种蛋白质.骨形态蛋白质是一种骨形态蛋白质.和是最重要的.细胞内膜网膜的内oplasmic网膜.这些都是短暂的过渡.翅膀的风化翅膀的风化

更多相关视频

Imaging Calcium in Drosophila at Egg Activation

Imaging Calcium in Drosophila at Egg Activation

Published on: August 6, 2016

Imaging Dpp Release from a Drosophila Wing Disc

Imaging Dpp Release from a Drosophila Wing Disc

Published on: October 30, 2019

相关概念视频

Feedback Regulation of Calcium Concentration

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...