合成和HER2特异性免疫SCIFI探针的体外评估
Katie Gristwood1, Saimir Luli2, Kenneth S Rankin3
1School of Natural and Environmental Sciences, Newcastle University, Bedson Building, Newcastle upon Tyne NE1 7RU, U.K.
ACS omega
|December 25, 2023
概括
一个新的免疫SCIFI探针,[89Zr]Zr-DFO-trastuzumab-BOD665,可以在现场进行自我激发的近红外光成像. 这种探测器显示了增强的组织透和对HER2生物标记物的特定结合,从而推进了分子成像.
科学领域:
- 生物医学光学成像技术
- 分子成像学分子成像学
- 放射化学 放射化学是指辐射化学.
背景情况:
- 二级Cerenkov诱导光成像 (SCIFI) 使用放射性同位素的Cerenkov发光激发光体进行深层组织成像.
- 双功能性免疫结合物为疾病生物标志物提供有针对性的传递和成像能力.
研究的目的:
- 合成和描述一种新的HER2特异性免疫SCIFI探针,用于自我激发的近红外光成像.
- 评估开发的immunoSCIFI探针的透深度和结合特异性.
主要方法:
- [89Zr]Zr-DFO-trastuzumab-BOD665的合成和特征,它是一种双功能的免疫结合物.
- 测量SCIFI信号透深度在注入血红蛋白的光学组织幻体中.
- 使用细胞测试对 SKBR3 和 MDA-MB-468 细胞进行比较,对 HER2 特定结合的评估.
主要成果:
- 免疫SCIFI探针在没有外部激发的情况下在现场产生近红外光.
- SCIFI信号的透深度是单独Cerenkov发光的2.05倍.
- 与MDA-MB-468细胞相比,探针对表达HER2的SKBR3细胞的结合明显更高.
结论:
- 开发的immunoSCIFI探针对HER2生物标志物的向分子成像显示出希望.
- 免疫SCIFI结构为疾病生物标志物查询提供了增强的组织透和特异性.
更多相关视频
11:34Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
Published on: December 5, 2017
12.6K
08:29Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
Published on: May 9, 2025
129
相关概念视频
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...