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微核细胞和与 Planorbidae 牛相关的细菌之间的共变

Camille Clerissi¹, Camille Huot², Anaïs Portet³

¹Current Affiliation: PSL Université Paris: EPHE-UPVD-CNRS, UAR 3278 CRIOBE, Université de Perpignan, Perpignan Cedex, France.

|December 25, 2023

概括

这项研究揭示了牛微生物群中微核细胞的隐藏多样性,使用了一种新的阻断原料. 分析了微核细胞和细菌群落,显示了一些牛物种的相关性和潜在的相互作用.

科学领域:

微生物学微生物学
细胞生态学细胞生态学
生物信息学是一种生物信息学.

背景情况:

微生物群落影响宿主健康,但微核细胞的多样性和与宿主中的细菌的相互作用尚不清楚.
与宏观生物相关的细菌得到了很好的研究,与微核细胞的有限知识形成鲜明对比.

研究的目的:

在Planorbidae牛中对微核细胞群体进行表征.
为了研究牛宿主中微核细胞和细菌群体之间的共变性.
开发一种研究宿主相关微生物群中的微核细胞多样性的方法.

主要方法:

开发了一种阻断原始物,以最大限度地减少在元编码过程中宿主DNA放大.
使用18S rRNA基因测序分析了微核细胞多样性.
使用16S rRNA基因测序分析了细菌多样性.
计算了两个微生物群体的α和β多样性.

主要成果:

微核细胞由三个类 (Amoebozoa,Opisthokonta,Alveolata) 占主导地位,而细菌在五个主要类中表现出更高的多样性.
微核细胞和细菌群体的组成在*Biomphalaria glabrata*中相关,但在*Planorbarius metidjensis*中没有相关.
网络分析揭示了共变的种群,这表明微核细胞和微核细胞之间的竞争对细菌的潜在自上而下的控制.

结论:

开发的阻断原料有助于研究以前隐藏的牛微生物群中的微核生物多样性.
这些发现突出了微核细胞对牛宿主内的细菌群体的影响的潜力.
需要进一步的研究来阐明这些微核细胞在宿主-微生物动态中的作用和相互作用.

关键词:

阻塞的初始化器异构分支 (Heterobranchia) 是一个不同分支的群体.这是一个全息生物.超标标的代码化微生物群中的微生物群

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