进行比较性分泌组分析,揭示了Pseudomonas plecoglossicida中的III型分泌系统和鞭毛组合之间的交叉对话
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A Visual Assay to Monitor T6SS-mediated Bacterial Competition
Published on: March 20, 2013
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Pan Qin1, Yingjia Luan1, Jinmei Yang1
1Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Heliyon
|December 25, 2023
概括
Pseudomonas plecoglossicida 使用第三类分泌系统 (T3SS) 进行感染. 删除T3SS转位子popB和popD会影响鞭毛,生物膜形成和细菌致病性.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 鱼类 水产养殖 水产养殖
背景情况:
- Pseudomonas plecoglossicida 在养殖鱼类中引起内脏颗粒瘤疾病,导致经济损失.
- 第三类分泌系统 (T3SS) 是许多格拉姆阴性细菌的关键毒性因素,但它们在P. plecoglossicida中的作用尚未完全理解.
研究的目的:
- 为了研究T3SS在P. plecoglossicida病原体中的功能.
- 阐明T3SS转位子 (PopB和PopD) 在细菌毒性中的作用.
主要方法:
- 构建一个T3SS转位器删除菌株 (△popBD) 的P. plecoglossicida.
- 使用无标签量化 (LFQ) 质谱法进行比较性秘密组分析.
- 试验验证鞭形态,粘附,移动性和生物膜形成的实验验证.
主要成果:
- 删除T3SS转位器popB和popD会对ExoU分泌,鞭毛组合和生物膜形成产生不利影响.
- PopB-PopD删除影响了P. plecoglossicida的鞭毛形态,粘附性,移动性和生物膜形成.
- 有证据表明,P. plecoglossicida T3SS及其鞭毛系统之间发生了交叉交谈.
结论:
- T3SS,特别是PopB-PopD转位器,在P. plecoglossicida的毒性中发挥着重要作用.
- 了解T3SS和鞭毛细胞之间的相互作用对于了解内脏颗粒瘤疾病的发病机理至关重要.
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