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探索葡萄种子和葡萄中不同分子重量,成分和结构的普朗索亚尼丁的分离模式和机制

Xiaoyi Chen¹, Hong Song¹, Shubo Zhou¹

¹College of Enology, Northwest A&F University, Yangling 712100, China.

Food chemistry: X

|December 25, 2023

概括

在Sephadex LH-20上分离高纯度的氨酸胺 (PAs) 取决于分子量和结构. 了解这些机制,包括像化PA这样的例外情况,可以提高净化效率.

科学领域:

自然产品化学自然产品化学
染色学分离科学染色学分离科学

背景情况:

氨酸 (PAs) 具有不同的生物功能,取决于它们的结构特征.
高纯度的PA对于功能研究至关重要,需要有效的分离技术.
塞法德克斯LH-20凝透色谱 (GPC) 是一种潜在的PA分离方法,但其潜在的机制尚未完全阐明.

研究的目的:

通过使用Sephadex LH-20来研究proanthocyanidins (PAs) 的分离模式和潜在机制.
阐明分子量,聚合度 (DP) 和结构特征对PA分离的影响.
建立准确分离不同PA组合的原则.

主要方法:

吸附动力模型用于确认Sephadex LH-20上的PA净化机制.
基于不同平均聚合度 (DP) 的PA分离的分析.
对特定PA成分 (单体,二元体等) 的分离原理的评估. ) 的情况.

主要成果:

增加的分子量/PA的平均DP与降低的极性,减少的键和增强的疏水效应相关,导致延迟化.
化PA首先被提取,无论其高DP,表明一种独特的相互作用.
确定了PA单体和二元体的特定分离原理.

结论:

这项研究阐明了控制Sephadex LH-20上PA分离的机制,突出了分子重量,DP和特定化学修饰的作用.
结果为优化PA净化效率和推进复杂自然产品精确分离技术提供了洞察力.
了解这些原则是释放PAs在各种应用中的全部潜力的关键.

关键词:

(+) - 甲基素 (PubChem CID9064) 的使用情况 (−) - 甲基因 (PubChem CID72276) 的使用情况 (−) - 甲基甲酸乙烯 (PubChem CID107905) 的使用情况.(−) - 甲基甲酸 (PubChem CID65064) 的使用情况吸附动力学吸附动力学氨基氨基胺是什么? 氨基胺是什么反向染色学是一种反向的染色学.塞法德克斯 LH-20-20 的情况.普克亚尼丁B1 (PubChem CID11250133) 的使用情况普克亚尼丁B2 (PubChem CID122738) 是一种可靠的化学物质.

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