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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Jul 7, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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染色校正多色多光子显微镜

Hugo Blanc1, Gabriel Kaddour2, Nicolas B David1

  • 1Laboratoire d'Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM, Institut Polytechnique de Paris, 91128 Palaiseau, France.

ACS photonics
|December 25, 2023
PubMed
概括
此摘要是机器生成的。

这项研究介绍了一种适应性策略,用于纠正多光子显微镜中的色态偏差. 这种技术使生物样品的清晰,同时的多色成像成为可能,增强了复杂过程的研究.

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相关实验视频

Last Updated: Jul 7, 2025

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Conducting Multiple Imaging Modes with One Fluorescence Microscope
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High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
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科学领域:

  • 生物医学光学 生物医学光学
  • 显微镜的使用方法
  • 细胞生物学 细胞生物学

背景情况:

  • 多光子显微镜使深层组织成像和生物过程的多色可视化成为可能.
  • 染色偏差,不同波长的空间转移,在多色多光子显微镜中降低了图像质量.
  • 现有的方法难以在整个视野中进行全面的色态偏差校正.

研究的目的:

  • 开发和验证一种适应性策略,用于纠正双束多光子显微镜中的轴向和侧向色态偏差.
  • 为了提高多色多光子成像中光束混合的性能和有效区域.
  • 为了实现生物标本的强大,高分辨率,同时多色成像.

主要方法:

  • 实施了折射相面罩,通过引入失焦来纠正轴向色差.
  • 在扫描过程中使用压电镜来动态补偿横向色谱偏差.
  • 测试了系统与斑马鱼胚胎和老鼠大脑切片的同时三色,两光子成像.

主要成果:

  • 为各种多光子目标在整个视野中实现了无的色彩校正.
  • 保持空间和时间分辨率,而不会影响图像质量.
  • 增加了梁混合工艺的有效面积超过一个数量级.

结论:

  • 拟议的适应性策略有效地纠正了多光子显微镜中的色谱偏差.
  • 这种方法为高质量的同时多色成像提供了强大而高效的解决方案.
  • 能够实现复杂的生物结构和过程的先进可视化 in vivo 和固定组织.