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通过维生素D缺乏的人类呼吸道上皮质增加ENaC介导的液体吸收

Emma M Stapleton¹, Andrew L Thurman¹, Alejandro A Pezzulo¹

¹Division of Pulmonary, Critical Care and Occupational Medicine, Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United States.

American journal of physiology. Cell physiology

|December 25, 2023

概括

维生素D缺乏会通过增加呼吸道上皮质中的ENaC活性,使呼吸道阻塞性疾病恶化. 这减少了气道表面的液体水分,可能导致粘液堵塞和呼吸道疾病.

科学领域:

肺部医学肺部医学
内分泌学在内分泌学.
皮质生物学皮质生物学

背景情况:

维生素D缺乏与阻塞性呼吸道疾病的更糟糕结果有关.
粘液脱水和堵塞是这些疾病的关键特征.
将维生素D缺乏与呼吸道病理联系在一起的确切机制尚不清楚.

研究的目的:

为了研究酸 (活性维生素D) 缺乏对人类呼吸道表皮的作用.
确定对离子通道活性的影响,特别是上皮质通道 (ENaC).
阐明维生素D在调节气道表面液体水化中的作用.

主要方法:

培养的人类呼吸道上皮质被用来研究酸缺乏症的影响.
对ENaC和Na-K子单位 (SCNN1G,ATP1B1) 的mRNA表达分析.
功能性检测包括Ussing室实验,测量氨酸敏感电流和液体吸收率.

主要成果:

酸缺乏症增加了SCNN1G和ATP1B1的mRNA水平.
缺少酸的表皮体表现出更快的液体吸收和增加对胺胺敏感电流,表明ENaC活性升高.
酸缺乏导致玛 (γ) ENaC蛋白水平增加,但不影响Na-K活动或ENaC内细胞分裂.

结论:

维生素D受体信号调节ENaC功能和气道表面液体吸收.
酸缺乏会增强ENaC的活性,导致气道水分的减少.
维生素D缺乏的ENaC活性增加可能会通过损害粘膜细胞运输和天生的免疫功能,导致呼吸道病理.

关键词:

在 ENaC 中.空气通道的空气通道.酸醇是一种酸.离子运输离子运输离子运输维生素-D 是一种维生素.

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