丰富了原生和重组的细胞外囊泡的菌根菌
Praapti Jayaswal1, Mohd Ilyas1, Kuljit Singh2
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster.
Journal of visualized experiments : JoVE
|December 25, 2023
概括
研究人员使用Mycobacterium smegmatis进行了重组的真菌细菌细胞外囊泡 (mEVs) 的工程设计. 这些工程 mEV 携带特定的蛋白质,为结核病提供了新型疫苗候选人的潜力.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
背景情况:
- 包括真菌菌在内的细菌自然产生细胞外囊泡 (EVs).
- 细菌EVs (bEVs) 含有多样化的细胞组件,使它们成为子单位疫苗的候选者.
- 重组EV被设计为表达特定的免疫原体,增强它们的疫苗潜力.
研究的目的:
- 在菌根菌中设计,构建和丰富复合菌根菌体EV (mEV).
- 为疫苗开发建立一个用于生成和净化工程 mEV 的协议.
主要方法:
- 使用了Mycobacterium smegmatis (Msm),一种恶毒的真菌菌,作为一个模型系统.
- 描述了本地MSM电动汽车的生成和丰富.
- 通过将记者蛋白 (mCherry) 或免疫原体 (EsxA) 融合到丰富的MsmEV蛋白 (Cfp-29) 进行工程重组mEV.
主要成果:
- 从Msm成功生成和丰富本地电动汽车.
- 构造的重组mEV表示mCherry或EsxA.
- 生产复合mEV的协议与本地EV生产相一致.
结论:
- 证明了一种可行的方法来创建重组的真菌菌体EVs.
- 这些经过工程设计的mEV为开发针对结核病等真菌细菌感染的新型疫苗策略提供了一个有前途的平台.
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