维生素D3通过直接调节淋巴腺类固醇激素合成†,促进鱼类卵细胞的发育
Gang Yang1, Ke Cheng1, Yanqing Huang2
1Department of Fishery Resources and Environment, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
Biology of reproduction
|December 25, 2023
概括
这项研究表明,维生素D3补充剂通过增强卵细胞成熟和激素合成来促进斑马鱼的卵巢发育. 最佳的维生素D3水平对于鱼类的生殖健康和维特洛根因生产至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 水产养殖营养水产养殖营养
背景情况:
- 维生素D受体和代谢酶存在于鱼的生殖腺中.
- 维生素D3在鱼卵巢发育中的特定作用仍然在很大程度上未被探索.
研究的目的:
- 研究食维生素D3对雌性斑马鱼卵巢发育的影响.
- 阐明维生素D3对鱼类繁殖的影响的分子机制.
主要方法:
- 斑马鱼被食不同度维生素D3 (0.700,1400,11200 IU/kg) 的饮食.
- 评估了卵巢发育,卵细胞成熟,激素水平 (雌激素,丸激素) 和维原蛋白.
- 鉴定了cyp19a1a促进体中的维生素D反应元素,并证明了其通过1.25(OH) 2D3的激活.
主要成果:
- 维生素D3缺乏 (0IU/kg) 导致卵巢毒性,包括间隙扩大和卵泡缩.
- 补充700和1400 IU/kg维生素D3显著改善了卵细胞成熟和上调的类固醇生成.
- 随着足够的维生素D3摄入,观察到血雌激素,丸激素和卵巢维特洛根因的升高.
结论:
- 适当的饮食维生素D3有助于斑马鱼的卵巢发育,并增强维特洛根因的合成.
- 维生素D受体/cyp19a1a/雌激素受体/vitellogenin (vdr/cyp19a1a/er/vtg) 基因轴参与维生素D3的作用.
- 这项研究强调了维生素D3在鱼类生殖生理学和水产养殖中的重要性.
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