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相关概念视频

Types of RNA01:23

Types of RNA

63.8K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.8K
Nucleic Acids02:43

Nucleic Acids

44.2K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
44.2K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Riboswitches01:56

Riboswitches

8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.4K
Nucleic Acid Structure01:25

Nucleic Acid Structure

6.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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相关实验视频

Updated: Jul 7, 2025

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

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RNet:用于预测RNA结合偏好的网络策略.

Haoquan Liu1, Yiren Jian2, Jinxuan Hou3

  • 1Institute of Biophysics and Department of Physics, Central China Normal University, Wuhan, 430079, China.

Briefings in bioinformatics
|December 25, 2023
PubMed
概括

预测RNA结合部位和动态对于设计有效的RNA抑制剂至关重要. 新的RNet方法准确地识别了结合点,并描述了动态行为,加速了药物发现.

关键词:
预测RNA结合部位的预测基于距离的图形算法界面绑定动态行为 界面绑定动态行为当地和全球网络属性.

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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

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An Assay for Quantifying Protein-RNA Binding in Bacteria
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An Assay for Quantifying Protein-RNA Binding in Bacteria

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相关实验视频

Last Updated: Jul 7, 2025

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

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An Assay for Quantifying Protein-RNA Binding in Bacteria
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科学领域:

  • 计算生物学 计算生物学
  • 生物信息学是一种生物信息学.
  • 药物发现 药物发现 药物发现

背景情况:

  • 由于复杂的相互作用和RNA灵活性,确定RNA结合偏好是具有挑战性的.
  • 目前的RNA抑制剂设计依赖于广泛的查,这是耗时和低效的.
  • 准确预测RNA结合部位和动态行为对于改善抑制剂设计成功率至关重要.

研究的目的:

  • 开发一种可解释的基于网络的方法,RNet,用于精确的RNA结合点和动态行为预测.
  • 为了提高RNA抑制剂设计的效率和准确性.
  • 为研究界提供关于RNA-蛋白相互作用的宝贵见解.

主要方法:

  • 开发了RNetsite,这是一种基于机器学习的网络分解算法,用于使用网络属性来预测RNA结合点.
  • 开发了RNetdyn,一种基于距离的动态图算法,用于分析抑制剂结合时的接口动态.
  • 专注于具有3D结构的大型RNA,不包括较小,更动态的调节性RNA.

主要成果:

  • RNetsite实现了高精度 (0.701在TE18,0.788在RB9),并证明了对RNA结构扰乱的稳定性.
  • 在对竞争性抑制剂的模拟测试中,RNetdyn的表现比传统方法高出30%.
  • 在基准测试中,RNet方法被证明是非常准确和稳健的.

结论:

  • 可解释的网络算法,RNet,准确预测RNA结合偏好和动态行为.
  • 通过提高预测准确性和效率,RNet显著加速RNA抑制剂设计.
  • 这种方法为RNA研究和治疗开发提供了宝贵的见解.